Density-dependent separation of encapsulated cells in a microfluidic channel by using a standing surface acoustic wave
- Authors
- Nam, Jeonghun; Lim, Hyunjung; Kim, Choong; Kang, Ji Yoon; Shin, Sehyun
- Issue Date
- 6월-2012
- Publisher
- AMER INST PHYSICS
- Keywords
- biological techniques; bioMEMS; cellular biophysics; flow separation; laminar flow; microchannel flow; surface acoustic waves
- Citation
- BIOMICROFLUIDICS, v.6, no.2
- Indexed
- SCIE
SCOPUS
- Journal Title
- BIOMICROFLUIDICS
- Volume
- 6
- Number
- 2
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/108317
- DOI
- 10.1063/1.4718719
- ISSN
- 1932-1058
- Abstract
- This study presents a method for density-based separation of monodisperse encapsulated cells using a standing surface acoustic wave (SSAW) in a microchannel. Even though monodisperse polymer beads can be generated by the state-of-the-art technology in microfluidics, the quantity of encapsulated cells cannot be controlled precisely. In the present study, mono-disperse alginate beads in a laminar flow can be separated based on their density using acoustophoresis. A mixture of beads of equal sizes but dissimilar densities was hydrodynamically focused at the entrance and then actively driven toward the sidewalls by a SSAW. The lateral displacement of a bead is proportional to the density of the bead, i.e., the number of encapsulated cells in an alginate bead. Under optimized conditions, the recovery rate of a target bead group (large-cell-quantity alginate beads) reached up to 97% at a rate of 2300 beads per minute. A cell viability test also confirmed that the encapsulated cells were hardly damaged by the acoustic force. Moreover, cell-encapsulating beads that were cultured for 1 day were separated in a similar manner. In conclusion, this study demonstrated that a SSAW can successfully separate monodisperse particles by their density. With the present technique for separating cell-encapsulating beads, the current cell engineering technology can be significantly advanced. (C) 2012 American Institute of Physics. [http://dx.doi.org/10.1063/1.4718719]
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Collections - College of Engineering > Department of Mechanical Engineering > 1. Journal Articles
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