Modulation of Alpha 1 Adrenergic Receptors on Urinary Bladder in Rat Spinal Cord Injury Model
DC Field | Value | Language |
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dc.contributor.author | Lee, Gilho | - |
dc.contributor.author | Park, Heeyoon | - |
dc.contributor.author | Park, Hong Suk | - |
dc.contributor.author | Lee, Jeong Gu | - |
dc.date.accessioned | 2021-09-06T19:22:41Z | - |
dc.date.available | 2021-09-06T19:22:41Z | - |
dc.date.created | 2021-06-18 | - |
dc.date.issued | 2012-06 | - |
dc.identifier.issn | 2093-4777 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/108325 | - |
dc.description.abstract | Purpose: Whereas many studies have focused on the vesical changes of the alpha 1 adrenergic receptor (AR) subtypes in partial outlet obstruction, few studies have addressed the modulation of the alpha 1 AR subtypes after spinal cord injury (SCI). Therefore, we studied the modulation of the alpha 1 ARs in urinary bladder in a rat SCI model. Methods: Four weeks after a SCI, the whole vesical bodies from eight female Sprague-Dawley rats and from eight controls were harvested. The total RNA was extracted from the samples and was used to prepare cDNA. We developed standard plasmid constructs of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and three alpha 1 ARs (alpha 1a, alpha 1b, and alpha 1d) to convert the cycle threshold (Ct) values from real-time polymerase chain reaction (RT-PCR) into subtype mRNA concentrations. The detected Ct values of 16 samples from RT-PCR were interpolated into the standard plasmid curves. Results: All serially diluted standard samples showed very good linearity. The mRNA expression of GAPDH was higher in the SCI group, whereas the mRNA expression of all alpha 1 ARs was lower in the SCI group than in the control animals. The alpha 1a, alpha 1b, and alpha 1d mRNA expression in the controls was 81.7%, 3.3%, and 15.1%, respectively, whereas the alpha 1a, alpha 1b, and alpha 1d mRNA expression in the SCI group was 33.5%, 5.2%, and 60.9%, respectively. Conclusions: SCI moderates the alpha 1 AR mRNA subtypes in the urinary bladder. The relatively increased alpha 1d or decreased alpha 1a AR mRNA expression may be a therapeutic candidate for controlling the symptoms of neurogenic bladder after SCI. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | KOREAN CONTINENCE SOC | - |
dc.title | Modulation of Alpha 1 Adrenergic Receptors on Urinary Bladder in Rat Spinal Cord Injury Model | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Jeong Gu | - |
dc.identifier.doi | 10.5213/inj.2012.16.2.62 | - |
dc.identifier.scopusid | 2-s2.0-84866942163 | - |
dc.identifier.wosid | 000209007400003 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL NEUROUROLOGY JOURNAL, v.16, no.2, pp.62 - 68 | - |
dc.relation.isPartOf | INTERNATIONAL NEUROUROLOGY JOURNAL | - |
dc.citation.title | INTERNATIONAL NEUROUROLOGY JOURNAL | - |
dc.citation.volume | 16 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 62 | - |
dc.citation.endPage | 68 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART001675966 | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Urology & Nephrology | - |
dc.relation.journalWebOfScienceCategory | Urology & Nephrology | - |
dc.subject.keywordAuthor | Spinal cord injuries | - |
dc.subject.keywordAuthor | Urinary bladder | - |
dc.subject.keywordAuthor | Alpha adrenergic receptors | - |
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