Modulation of Alpha 1 Adrenergic Receptors on Urinary Bladder in Rat Spinal Cord Injury Model
- Authors
- Lee, Gilho; Park, Heeyoon; Park, Hong Suk; Lee, Jeong Gu
- Issue Date
- 6월-2012
- Publisher
- KOREAN CONTINENCE SOC
- Keywords
- Spinal cord injuries; Urinary bladder; Alpha adrenergic receptors
- Citation
- INTERNATIONAL NEUROUROLOGY JOURNAL, v.16, no.2, pp.62 - 68
- Indexed
- SCOPUS
KCI
- Journal Title
- INTERNATIONAL NEUROUROLOGY JOURNAL
- Volume
- 16
- Number
- 2
- Start Page
- 62
- End Page
- 68
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/108325
- DOI
- 10.5213/inj.2012.16.2.62
- ISSN
- 2093-4777
- Abstract
- Purpose: Whereas many studies have focused on the vesical changes of the alpha 1 adrenergic receptor (AR) subtypes in partial outlet obstruction, few studies have addressed the modulation of the alpha 1 AR subtypes after spinal cord injury (SCI). Therefore, we studied the modulation of the alpha 1 ARs in urinary bladder in a rat SCI model. Methods: Four weeks after a SCI, the whole vesical bodies from eight female Sprague-Dawley rats and from eight controls were harvested. The total RNA was extracted from the samples and was used to prepare cDNA. We developed standard plasmid constructs of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and three alpha 1 ARs (alpha 1a, alpha 1b, and alpha 1d) to convert the cycle threshold (Ct) values from real-time polymerase chain reaction (RT-PCR) into subtype mRNA concentrations. The detected Ct values of 16 samples from RT-PCR were interpolated into the standard plasmid curves. Results: All serially diluted standard samples showed very good linearity. The mRNA expression of GAPDH was higher in the SCI group, whereas the mRNA expression of all alpha 1 ARs was lower in the SCI group than in the control animals. The alpha 1a, alpha 1b, and alpha 1d mRNA expression in the controls was 81.7%, 3.3%, and 15.1%, respectively, whereas the alpha 1a, alpha 1b, and alpha 1d mRNA expression in the SCI group was 33.5%, 5.2%, and 60.9%, respectively. Conclusions: SCI moderates the alpha 1 AR mRNA subtypes in the urinary bladder. The relatively increased alpha 1d or decreased alpha 1a AR mRNA expression may be a therapeutic candidate for controlling the symptoms of neurogenic bladder after SCI.
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