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High-Performance Liquid Chromatography-Based Multivariate Analysis to Predict the Estrogenic Activity of an Epimedium koreanum Extract

Authors
Islam, M. NurulKim, UnyongKim, Dong-HyunDong, Mi-SookYoo, Hye Hyun
Issue Date
5월-2012
Publisher
TAYLOR & FRANCIS LTD
Keywords
Epimedium koreanum Nakai; high-performance liquid chromatography (HPLC); multi-variate analysis; estrogenic activity
Citation
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, v.76, no.5, pp.923 - 927
Indexed
SCIE
SCOPUS
Journal Title
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
Volume
76
Number
5
Start Page
923
End Page
927
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/108504
DOI
10.1271/bbb.110922
ISSN
0916-8451
Abstract
This study characterizes the correlation between the chemical fingerprint and estrogenic activity of an Epimedium koreanum extract. The estrogenic activity of 31 E. koreanum extract samples was evaluated by a luciferase reporter gene assay, and the samples were classified into 3 groups based on their bioactivity. A chemical fingerprint analysis was performed on each sample by high-performance liquid chromatography (HPLC), and 44 common peaks were selected from the chromatogram and used as a dataset for a pattern recognition analysis. A canonical discriminant analysis performed on this dataset determined a distinct distribution of the samples according to their estrogenic activity on the scoring plot. The classification results showed that 90.3% of the original grouped cases had been correctly classified. The total content of the 4 major extract compounds, epimedin A, epimedin B, epimedin C, and icariin, exhibited good correlation (r = 0.784) with the estrogenic activities of the respective extracts. This chromatographic fingerprint-chemometric analysis system could be useful for predicting the E. koreanum pharmacological activity and consequent biological activity-relevant quality control assessment.
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