Identification and biocontrol efficacy of Streptomyces miharaensis producing filipin III against Fusarium wilt
- Authors
- Kim, Jeong Do; Han, Jae Woo; Hwang, In Cheon; Lee, Dongho; Kim, Beom Seok
- Issue Date
- 4월-2012
- Publisher
- WILEY
- Keywords
- Streptomyces miharaensis; Antagonistic bacteria; Fusarium wilt; Filipin III; Biofungicides
- Citation
- JOURNAL OF BASIC MICROBIOLOGY, v.52, no.2, pp.150 - 159
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF BASIC MICROBIOLOGY
- Volume
- 52
- Number
- 2
- Start Page
- 150
- End Page
- 159
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/108796
- DOI
- 10.1002/jobm.201100134
- ISSN
- 0233-111X
- Abstract
- A number of bacterial strains were isolated from the internal tissue of Trapa japonica. Of these, strain KPE62302H, which had a 16S rDNA sequence identical to that of Streptomyces miharaensis showed antifungal activity against several plant pathogens. Treatment of seeds with strain KPE62302H induced a significant reduction in the incidence of Fusarium wilt in tomato plants compared with untreated controls. An antifungal substance (FP-1) was purified from the culture extract of strain KPE62302H using C18 flash and Sephadex LH-20 column chromatography and reverse phase HPLC. Extensive spectrometric analysis using MS and NMR identified this as filipin III. FP-1 inhibited the mycelial growth of plant pathogenic fungi such as Alternaria mali, Aspergillus niger, Colletotrichum gloeosporioides, C. orbiculare, Cylindrocarpon destructans, Diaporthe citiri, Fusarium oxysporum at 1-0 mu g ml(-1) and also markedly inhibited the development of Fusarium wilt caused by F. oxysporum f.sp. lycopersici in tomato plants by treatment with 10 mu g ml(-1) under greenhouse conditions. The efficacy of FP-1 against Fusarium wilt was comparable to that of the synthetic fungicide benomyl. An egfp-tagged strain of KPE62302H confirmed its ability to colonize tomato plants.
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Collections - College of Life Sciences and Biotechnology > Division of Biotechnology > 1. Journal Articles
- Graduate School > Department of Plant Biotechnology > 1. Journal Articles
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