New Method of Manganese-Enhanced Magnetic Resonance Imaging (MEMRI) for Rat Brain Research
- Authors
- Jeong, Keun-Yeong; Lee, Chulhyun; Cho, Jee-Hyun; Kang, Ji-Hyuk; Na, Heung-Sik
- Issue Date
- 4월-2012
- Publisher
- INT PRESS EDITING CENTRE INC
- Keywords
- cannula implantation; intracerebroventricular injection; manganese-enhanced MRI; Mn2+; rat brain
- Citation
- EXPERIMENTAL ANIMALS, v.61, no.2, pp.157 - 164
- Indexed
- SCIE
SCOPUS
- Journal Title
- EXPERIMENTAL ANIMALS
- Volume
- 61
- Number
- 2
- Start Page
- 157
- End Page
- 164
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/108827
- DOI
- 10.1538/expanim.61.157
- ISSN
- 1341-1357
- Abstract
- Manganese (Mn2+)-enhanced MRI (MEMRI) is known to provide insight into functional and anatomical biology. However, this method, which uses Mn2+ as a MRI-detectable contrast agent, has drawbacks such as the toxicity to cells beyond a certain level of Mn2+. In this study, we attempt to determine a new method of ICV administration, the optimal concentration of administered Mn2+ and the optimal MEMRI acquisition time following administration. Male Sprague-Dawley rats were used in the following experimental sessions: (1) intracerebroventricular (ICV) cannula implantation in the region of the cisterna magna, (2) serial dilution of MnCl2 (20-80 mM), (3) ICV administration of MnCl2 through the cannula, and (4) T-1-weighted MRI measurements. We confirmed that cannula implantation in the region of the cisterna magna was a new ICV injection method for the administration of a contrast agent. The optimal concentration for MEMRI was 20/50 mM/mu l of MnCl2. The MEMRI data acquired at different time points indicate that most signal enhancement is maintained during 14-48 h after contrast agent injection, and 24 h was the optimal time to acquire images of the rat brain. The present study offers optimized parameters for contrast agent injection that would be a good basis for studies using MEMRI to research the rat brain.
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