Annulus Fibrosus Cells Interact With Neuron-Like Cells to Modulate Production of Growth Factors and Cytokines in Symptomatic Disc Degeneration
DC Field | Value | Language |
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dc.contributor.author | Moon, Hong Joo | - |
dc.contributor.author | Kim, Joo Han | - |
dc.contributor.author | Lee, Hack Sun | - |
dc.contributor.author | Chotai, Silky | - |
dc.contributor.author | Kang, James D. | - |
dc.contributor.author | Suh, Jung Keun | - |
dc.contributor.author | Park, Youn-Kwan | - |
dc.date.accessioned | 2021-09-06T23:14:55Z | - |
dc.date.available | 2021-09-06T23:14:55Z | - |
dc.date.created | 2021-06-18 | - |
dc.date.issued | 2012-01-01 | - |
dc.identifier.issn | 0362-2436 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/109114 | - |
dc.description.abstract | Study Design. We hypothesized that AF/neuron interactions during annular injury were involved in neovascularization and nerve ingrowth, the pathologic hallmarks of symptomatic disc degeneration. Objective. To identify growth factors and inflammatory cytokines related to AF/neuron interactions using in vitro model. Summary of Background Data. Discogenic pain is the chronic intractable pain initiated by tears in the outer annulus fibrosus (AF); this is a unique structure with free nerve endings at outer one-third, located beside dorsal root ganglia. The relationship between AF and neuron cells in annular injury has not been extensively investigated. Methods. Human AF cells were cocultured with a retinoic acid (RA)-treated SH-SY5Y human neuroblastoma cell line (neuron-like cells). Conditioned media from cells cultured alone or in coculture were assayed for growth factors and inflammatory cytokines using enzyme-linked immunosorbent assays. The responses of the neuron-like cells, the AF cells, and the cocultured group to IL-1 beta/TNF-alpha were compared using the same outcome measures. Results. RA-treated SH-SY5Y cells showed significant neurite outgrowth on the 7th day; this is a typical morphologic finding of neuron-like cells. Neuron-like cells produced vascular endothelial growth factor (VEGF) and IGF-1 under basal conditions and dose-dependently secreted small amounts of IL-8 in response to TNF-alpha. Coculturing enhanced the secretion of VEGF, TGF-beta 1, and beta-NGF, and suppressed the production of IGF-1. VEGF in the coculture group and the AF cells was downregulated by IL-1 beta/TNF-alpha stimulation. IL-1 beta/TNF-alpha stimulation enhanced the production of large amounts of IL-6 and IL-8 from AF cells; IL-1 beta produced a greater response than TNF-alpha. The neuron-like cells did not produce detectable amounts of IL-6 or IL-8. Conclusion. These studies suggest that AF cells are involved in an inflammatory reaction and that the interactions between AF and neuron-like cells enhance the production of growth factors responsible for neovascularization and nerve ingrowth. AF injury has the potential to initiate neovascularization/nerve ingrowth and an inflammatory reaction through the interactions of AF and neural tissues. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | LIPPINCOTT WILLIAMS & WILKINS | - |
dc.subject | LOW-BACK-PAIN | - |
dc.subject | VASCULAR-PERMEABILITY FACTOR | - |
dc.subject | AMYLOID-BETA-PEPTIDE | - |
dc.subject | NERVE GROWTH | - |
dc.subject | INTERVERTEBRAL DISC | - |
dc.subject | HUMAN NEUROBLASTOMA | - |
dc.subject | RETINOIC ACID | - |
dc.subject | SH-SY5Y CELLS | - |
dc.subject | IN-VITRO | - |
dc.subject | DIFFERENTIATION | - |
dc.title | Annulus Fibrosus Cells Interact With Neuron-Like Cells to Modulate Production of Growth Factors and Cytokines in Symptomatic Disc Degeneration | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Joo Han | - |
dc.contributor.affiliatedAuthor | Suh, Jung Keun | - |
dc.contributor.affiliatedAuthor | Park, Youn-Kwan | - |
dc.identifier.doi | 10.1097/BRS.0b013e31820cd2d8 | - |
dc.identifier.scopusid | 2-s2.0-84655176540 | - |
dc.identifier.wosid | 000298410500014 | - |
dc.identifier.bibliographicCitation | SPINE, v.37, no.1, pp.2 - 9 | - |
dc.relation.isPartOf | SPINE | - |
dc.citation.title | SPINE | - |
dc.citation.volume | 37 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 2 | - |
dc.citation.endPage | 9 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Neurosciences & Neurology | - |
dc.relation.journalResearchArea | Orthopedics | - |
dc.relation.journalWebOfScienceCategory | Clinical Neurology | - |
dc.relation.journalWebOfScienceCategory | Orthopedics | - |
dc.subject.keywordPlus | LOW-BACK-PAIN | - |
dc.subject.keywordPlus | VASCULAR-PERMEABILITY FACTOR | - |
dc.subject.keywordPlus | AMYLOID-BETA-PEPTIDE | - |
dc.subject.keywordPlus | NERVE GROWTH | - |
dc.subject.keywordPlus | INTERVERTEBRAL DISC | - |
dc.subject.keywordPlus | HUMAN NEUROBLASTOMA | - |
dc.subject.keywordPlus | RETINOIC ACID | - |
dc.subject.keywordPlus | SH-SY5Y CELLS | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordAuthor | AF cells | - |
dc.subject.keywordAuthor | cytokines | - |
dc.subject.keywordAuthor | growth factors | - |
dc.subject.keywordAuthor | neovascularization | - |
dc.subject.keywordAuthor | nerve ingrowth | - |
dc.subject.keywordAuthor | SH-SY5Y cell line | - |
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