Chromosomal cephalosporinase in Enterobacter hormaechei as an ancestor of ACT-1 plasmid-mediated AmpC beta-lactamase
- Authors
- Roh, Kyoung Ho; Song, Wonkeun; Chung, Hae-Sun; Lee, Yang Soon; Yum, Jong Hwa; Yi, Ha Na; Chun, Jong Sik; Yong, Dongeun; Lee, Kyungwon; Chong, Yunsop
- Issue Date
- 1월-2012
- Publisher
- SOC GENERAL MICROBIOLOGY
- Citation
- JOURNAL OF MEDICAL MICROBIOLOGY, v.61, no.1, pp.94 - 100
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF MEDICAL MICROBIOLOGY
- Volume
- 61
- Number
- 1
- Start Page
- 94
- End Page
- 100
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/109164
- DOI
- 10.1099/jmm.0.032573-0
- ISSN
- 0022-2615
- Abstract
- In this study of the diversity of AmpC beta-lactamase in clinical isolates of Enterobacter spp., a strain was found carrying the plasmid-mediated AmpC beta-lactamase ACT-1 gene on its chromosome. The strain was identified as Enterobacter hormaechei using phylogenetic analysis of 16S rRNA and hsp60 genes. In addition, the species was confirmed by DNA DNA hybridization. The genetic environment of the bla(ACT-1) gene was characterized, including the ampR and ampG genes, using a two-step PCR. The amino acid sequences of AmpR at serine 35, arginine 86, glycine 102, aspartic acid 135 and tyrosine 264 were conserved. Measurement of the transcription level of the bla(ACT-1), gene using real-time quantitative PCR showed that it increased 1.98-fold following cefoxitin induction. These results suggest that the plasmid-mediated bla(ACT-1) gene originated from the chromosome of E. hormaechei.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Medicine > Department of Medical Science > 1. Journal Articles
- College of Health Sciences > School of Biosystems and Biomedical Sciences > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.