Enzyme-conjugated ZnO nanocrystals for collisional quenching-based glucose sensing
DC Field | Value | Language |
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dc.contributor.author | Kim, Ki-Eun | - |
dc.contributor.author | Kim, Tae Geun | - |
dc.contributor.author | Sung, Yun-Mo | - |
dc.date.accessioned | 2021-09-07T00:02:45Z | - |
dc.date.available | 2021-09-07T00:02:45Z | - |
dc.date.created | 2021-06-18 | - |
dc.date.issued | 2012 | - |
dc.identifier.issn | 1466-8033 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/109365 | - |
dc.description.abstract | A simple approach to sensitive glucose detection has been developed based upon variation in the fluorescence of ZnO nanocrystals with glucose concentration. ZnO nanocrystals were successfully synthesized in wurtzite structure using a surfactant, mercaptoundecanoic acid (MUA) via the polyol method. MUA molecules not only served as a template for the synthesis of spherical-shape nanoparticles but also provided water solubility and biocompatibility due to its carboxyl group. Carboxyl-terminated ZnO nanocrystals were activated by esterification of n-hydroxysulfosuccinimide (Sulfo-NHS) catalyzed by water-soluble 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC). Glucose oxidase (GOx), an enzyme could be immobilized to ZnO nanocrystals by replacing NHS with amino-acid groups of GOx. ZnO-MUA-GOx bioconjugates showed a decrease in the photoluminescence (PL) intensity by appearance of glucose molecules due to the collisional quenching by hydrogen peroxide generated from enzymatic oxidation reaction of glucose. PL intensity showed a linear decrease with glucose concentration from 1.6 to 33.3 mM, which fully covers the physiological glucose level. ZnO-MUA-GOx bioconjugates showed a detection limit lower than 0.33 mM and a response time less than 5 s. They also revealed distinct specificity against cholesterol molecules. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ROYAL SOC CHEMISTRY | - |
dc.subject | FLUORESCENCE DETECTION | - |
dc.subject | SURFACE MODIFICATION | - |
dc.subject | QUANTUM DOTS | - |
dc.subject | NANOPARTICLE | - |
dc.subject | ELECTRON | - |
dc.subject | BIOSENSOR | - |
dc.subject | OXIDASE | - |
dc.subject | SHELL | - |
dc.title | Enzyme-conjugated ZnO nanocrystals for collisional quenching-based glucose sensing | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Tae Geun | - |
dc.contributor.affiliatedAuthor | Sung, Yun-Mo | - |
dc.identifier.doi | 10.1039/c2ce06410c | - |
dc.identifier.scopusid | 2-s2.0-84860286000 | - |
dc.identifier.wosid | 000301735400032 | - |
dc.identifier.bibliographicCitation | CRYSTENGCOMM, v.14, no.8, pp.2859 - 2865 | - |
dc.relation.isPartOf | CRYSTENGCOMM | - |
dc.citation.title | CRYSTENGCOMM | - |
dc.citation.volume | 14 | - |
dc.citation.number | 8 | - |
dc.citation.startPage | 2859 | - |
dc.citation.endPage | 2865 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Crystallography | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Crystallography | - |
dc.subject.keywordPlus | FLUORESCENCE DETECTION | - |
dc.subject.keywordPlus | SURFACE MODIFICATION | - |
dc.subject.keywordPlus | QUANTUM DOTS | - |
dc.subject.keywordPlus | NANOPARTICLE | - |
dc.subject.keywordPlus | ELECTRON | - |
dc.subject.keywordPlus | BIOSENSOR | - |
dc.subject.keywordPlus | OXIDASE | - |
dc.subject.keywordPlus | SHELL | - |
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