Cigarette smoke-induced early growth response-1 regulates the expression of the cysteine-rich 61 in human skin dermal fibroblasts
DC Field | Value | Language |
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dc.contributor.author | Kim, Ji Na | - |
dc.contributor.author | Kim, Hee Joo | - |
dc.contributor.author | Jeong, Sang Hoon | - |
dc.contributor.author | Kye, Young Chul | - |
dc.contributor.author | Son, Sang Wook | - |
dc.date.accessioned | 2021-09-07T05:33:06Z | - |
dc.date.available | 2021-09-07T05:33:06Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2011-12 | - |
dc.identifier.issn | 0906-6705 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/111005 | - |
dc.description.abstract | Tobacco smoke is known to be an element contributing to accelerate premature skin ageing. Cysteine-rich 61 (Cyr61) is a member of the connective tissue factor CCN (Cyr61, CTGF and Nov) family, and early growth response-1 (Egr-1) is a generally expressed member of the zinc-finger family of transcription factors. To investigate the regulatory potential of Egr-1 on expression of Cyr61 by smoking, this study examined the hypothesis that cigarette smoke-induced Egr-1 induces expression of Cyr61 in human skin dermal fibroblasts (HSDF). HSDF were exposed to different concentrations of cigarette smoke extract (CSE) for 24 h; a cytotoxicity assay was then performed for the detection of cell proliferation. Results of Western blot and reverse transcription-polymerase chain reaction (RT-PCR) showed that CSE induces a transient synthesis of Egr-1 in HSDF. Cyr61 mRNA and protein levels showed a marked increase in a time-dependent manner after CSE exposure. Following transfection with an Egr-1 overexpression vector, HSDF showed increased activity of the Cyr61 promoter in a dose-dependent manner. Using Egr-1 interfering RNA, we confirmed that CSE-induced Cyr61 expression was dependent on Egr-1 expression. Findings of this study indicate that Egr-1-dependent induction of Cyr61 may contribute to premature skin ageing by smoking. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | WILEY | - |
dc.subject | HUMAN-LUNG FIBROBLASTS | - |
dc.subject | FACTOR-BETA | - |
dc.subject | MATRIX METALLOPROTEINASE-1 | - |
dc.subject | FUNCTIONAL-PROPERTIES | - |
dc.subject | GENE-EXPRESSION | - |
dc.subject | EGR-1 | - |
dc.subject | CYR61 | - |
dc.subject | INHIBITOR | - |
dc.subject | IMMEDIATE | - |
dc.subject | SEQUENCES | - |
dc.title | Cigarette smoke-induced early growth response-1 regulates the expression of the cysteine-rich 61 in human skin dermal fibroblasts | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kye, Young Chul | - |
dc.contributor.affiliatedAuthor | Son, Sang Wook | - |
dc.identifier.doi | 10.1111/j.1600-0625.2011.01380.x | - |
dc.identifier.scopusid | 2-s2.0-81455141243 | - |
dc.identifier.wosid | 000297154000006 | - |
dc.identifier.bibliographicCitation | EXPERIMENTAL DERMATOLOGY, v.20, no.12, pp.992 - 997 | - |
dc.relation.isPartOf | EXPERIMENTAL DERMATOLOGY | - |
dc.citation.title | EXPERIMENTAL DERMATOLOGY | - |
dc.citation.volume | 20 | - |
dc.citation.number | 12 | - |
dc.citation.startPage | 992 | - |
dc.citation.endPage | 997 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Dermatology | - |
dc.relation.journalWebOfScienceCategory | Dermatology | - |
dc.subject.keywordPlus | HUMAN-LUNG FIBROBLASTS | - |
dc.subject.keywordPlus | FACTOR-BETA | - |
dc.subject.keywordPlus | MATRIX METALLOPROTEINASE-1 | - |
dc.subject.keywordPlus | FUNCTIONAL-PROPERTIES | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | EGR-1 | - |
dc.subject.keywordPlus | CYR61 | - |
dc.subject.keywordPlus | INHIBITOR | - |
dc.subject.keywordPlus | IMMEDIATE | - |
dc.subject.keywordPlus | SEQUENCES | - |
dc.subject.keywordAuthor | cigarette smoke | - |
dc.subject.keywordAuthor | cysteine-rich 61 | - |
dc.subject.keywordAuthor | early growth response-1 | - |
dc.subject.keywordAuthor | skin ageing | - |
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