Cigarette smoke-induced early growth response-1 regulates the expression of the cysteine-rich 61 in human skin dermal fibroblasts
- Authors
- Kim, Ji Na; Kim, Hee Joo; Jeong, Sang Hoon; Kye, Young Chul; Son, Sang Wook
- Issue Date
- 12월-2011
- Publisher
- WILEY
- Keywords
- cigarette smoke; cysteine-rich 61; early growth response-1; skin ageing
- Citation
- EXPERIMENTAL DERMATOLOGY, v.20, no.12, pp.992 - 997
- Indexed
- SCIE
SCOPUS
- Journal Title
- EXPERIMENTAL DERMATOLOGY
- Volume
- 20
- Number
- 12
- Start Page
- 992
- End Page
- 997
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/111005
- DOI
- 10.1111/j.1600-0625.2011.01380.x
- ISSN
- 0906-6705
- Abstract
- Tobacco smoke is known to be an element contributing to accelerate premature skin ageing. Cysteine-rich 61 (Cyr61) is a member of the connective tissue factor CCN (Cyr61, CTGF and Nov) family, and early growth response-1 (Egr-1) is a generally expressed member of the zinc-finger family of transcription factors. To investigate the regulatory potential of Egr-1 on expression of Cyr61 by smoking, this study examined the hypothesis that cigarette smoke-induced Egr-1 induces expression of Cyr61 in human skin dermal fibroblasts (HSDF). HSDF were exposed to different concentrations of cigarette smoke extract (CSE) for 24 h; a cytotoxicity assay was then performed for the detection of cell proliferation. Results of Western blot and reverse transcription-polymerase chain reaction (RT-PCR) showed that CSE induces a transient synthesis of Egr-1 in HSDF. Cyr61 mRNA and protein levels showed a marked increase in a time-dependent manner after CSE exposure. Following transfection with an Egr-1 overexpression vector, HSDF showed increased activity of the Cyr61 promoter in a dose-dependent manner. Using Egr-1 interfering RNA, we confirmed that CSE-induced Cyr61 expression was dependent on Egr-1 expression. Findings of this study indicate that Egr-1-dependent induction of Cyr61 may contribute to premature skin ageing by smoking.
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Collections - College of Medicine > Department of Medical Science > 1. Journal Articles
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