The Effect of Doxycycline on PMA-Induced MUC5B Expression via MMP-9 and p38 in NCI-H292 Cells
DC Field | Value | Language |
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dc.contributor.author | Bae, Chang Hoon | - |
dc.contributor.author | Chen, Seung Min | - |
dc.contributor.author | Lee, Heung-Man | - |
dc.contributor.author | Song, Si-Youn | - |
dc.contributor.author | Kim, Yong-Dae | - |
dc.date.accessioned | 2021-09-07T05:44:04Z | - |
dc.date.available | 2021-09-07T05:44:04Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2011-12 | - |
dc.identifier.issn | 1976-8710 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/111066 | - |
dc.description.abstract | Objectives. Doxycycline is commonly used in medicine for its bacteriostatic antimicrobial properties. Recent studies have reported that doxycycline also has anti-inflammatory effects. Matrix metalloproteinase (MMP)-9 has been found to be involved in the physiological and pathological process of inflammatory airway disease. Phorbol 12-myristate 13-acetate (PMA), a protein kinase C activator, is known to stimulate the expression of MMP and mucin genes in the airway and intestinal epithelial cells, Therefore, the effects and signal pathways of doxycycline on PMA-induced MUC5B expression dependent MMP-9 in human airway epithelial cells were investigated. Methods. In human NCI-H292 airway epithelial cells, MUC5B and MMP-9 mRNA expression, MUC5B protein expression, and MMP-9 protein activity after the treatment with PMA, MMP-9 or doxycycline were determined by reverse transcriptase-polymerase chain reaction, enzyme immunoassay, gelatin zymography, and Western blot analysis. Results. PMA increased MMP-9 and MUC5B expression. MMP-9 increased MUC5B expression. Doxycycline inhibited PMA-induced MUC5B expression, and PMA-induced MMP-9 mRNA expression and protein activity Doxycycline inhibited phosphorylation of p38 induced by PMA and MMP-9. Conclusion. The results of this study suggest that doxycycline inhibited PMA-induced MUC5B mRNA expression and protein production through the MMP-9 and p38 pathways in human NCI-H292 airway epithelial cells. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | KOREAN SOC OTORHINOLARYNGOL | - |
dc.subject | INDUCED MUCIN PRODUCTION | - |
dc.subject | GENE-EXPRESSION | - |
dc.subject | MATRIX-METALLOPROTEINASE-9 | - |
dc.subject | ERK | - |
dc.title | The Effect of Doxycycline on PMA-Induced MUC5B Expression via MMP-9 and p38 in NCI-H292 Cells | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Heung-Man | - |
dc.identifier.doi | 10.3342/ceo.2011.4.4.177 | - |
dc.identifier.scopusid | 2-s2.0-84455174642 | - |
dc.identifier.wosid | 000298532700004 | - |
dc.identifier.bibliographicCitation | CLINICAL AND EXPERIMENTAL OTORHINOLARYNGOLOGY, v.4, no.4, pp.177 - 183 | - |
dc.relation.isPartOf | CLINICAL AND EXPERIMENTAL OTORHINOLARYNGOLOGY | - |
dc.citation.title | CLINICAL AND EXPERIMENTAL OTORHINOLARYNGOLOGY | - |
dc.citation.volume | 4 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 177 | - |
dc.citation.endPage | 183 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART001613945 | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.description.journalRegisteredClass | other | - |
dc.relation.journalResearchArea | Otorhinolaryngology | - |
dc.relation.journalWebOfScienceCategory | Otorhinolaryngology | - |
dc.subject.keywordPlus | INDUCED MUCIN PRODUCTION | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | MATRIX-METALLOPROTEINASE-9 | - |
dc.subject.keywordPlus | ERK | - |
dc.subject.keywordAuthor | Doxycycline | - |
dc.subject.keywordAuthor | Inflammation | - |
dc.subject.keywordAuthor | Phorbol myristate acetate | - |
dc.subject.keywordAuthor | Matrix metalloproteinase-9 | - |
dc.subject.keywordAuthor | p38 | - |
dc.subject.keywordAuthor | Mucins | - |
dc.subject.keywordAuthor | MUC5B | - |
dc.subject.keywordAuthor | Epithelial cells | - |
dc.subject.keywordAuthor | NCI-H292 cell | - |
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