The Effect of Doxycycline on PMA-Induced MUC5B Expression via MMP-9 and p38 in NCI-H292 Cells
- Authors
- Bae, Chang Hoon; Chen, Seung Min; Lee, Heung-Man; Song, Si-Youn; Kim, Yong-Dae
- Issue Date
- 12월-2011
- Publisher
- KOREAN SOC OTORHINOLARYNGOL
- Keywords
- Doxycycline; Inflammation; Phorbol myristate acetate; Matrix metalloproteinase-9; p38; Mucins; MUC5B; Epithelial cells; NCI-H292 cell
- Citation
- CLINICAL AND EXPERIMENTAL OTORHINOLARYNGOLOGY, v.4, no.4, pp.177 - 183
- Indexed
- SCIE
SCOPUS
KCI
OTHER
- Journal Title
- CLINICAL AND EXPERIMENTAL OTORHINOLARYNGOLOGY
- Volume
- 4
- Number
- 4
- Start Page
- 177
- End Page
- 183
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/111066
- DOI
- 10.3342/ceo.2011.4.4.177
- ISSN
- 1976-8710
- Abstract
- Objectives. Doxycycline is commonly used in medicine for its bacteriostatic antimicrobial properties. Recent studies have reported that doxycycline also has anti-inflammatory effects. Matrix metalloproteinase (MMP)-9 has been found to be involved in the physiological and pathological process of inflammatory airway disease. Phorbol 12-myristate 13-acetate (PMA), a protein kinase C activator, is known to stimulate the expression of MMP and mucin genes in the airway and intestinal epithelial cells, Therefore, the effects and signal pathways of doxycycline on PMA-induced MUC5B expression dependent MMP-9 in human airway epithelial cells were investigated. Methods. In human NCI-H292 airway epithelial cells, MUC5B and MMP-9 mRNA expression, MUC5B protein expression, and MMP-9 protein activity after the treatment with PMA, MMP-9 or doxycycline were determined by reverse transcriptase-polymerase chain reaction, enzyme immunoassay, gelatin zymography, and Western blot analysis. Results. PMA increased MMP-9 and MUC5B expression. MMP-9 increased MUC5B expression. Doxycycline inhibited PMA-induced MUC5B expression, and PMA-induced MMP-9 mRNA expression and protein activity Doxycycline inhibited phosphorylation of p38 induced by PMA and MMP-9. Conclusion. The results of this study suggest that doxycycline inhibited PMA-induced MUC5B mRNA expression and protein production through the MMP-9 and p38 pathways in human NCI-H292 airway epithelial cells.
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