Restoration of autophagy by puerarin in ethanol-treated hepatocytes via the activation of AMP-activated protein kinase
DC Field | Value | Language |
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dc.contributor.author | Noh, Byung-Kyu | - |
dc.contributor.author | Lee, Jung Kyu | - |
dc.contributor.author | Jun, Hee-jin | - |
dc.contributor.author | Lee, Ji Hae | - |
dc.contributor.author | Jia, Yaoyao | - |
dc.contributor.author | Hoang, Minh-Hien | - |
dc.contributor.author | Kim, Jung-Wan | - |
dc.contributor.author | Park, Kwan-Hwa | - |
dc.contributor.author | Lee, Sung-Joon | - |
dc.date.accessioned | 2021-09-07T07:04:51Z | - |
dc.date.available | 2021-09-07T07:04:51Z | - |
dc.date.created | 2021-06-18 | - |
dc.date.issued | 2011-10-22 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/111354 | - |
dc.description.abstract | We investigated the effects of puerarin, the major isoflavone in Kudzu roots, on the regulation of autophagy in ethanol-treated hepatocytes. Incubation in ethanol (100 mM) for 24 h reduced cell viability by 20% and increased the cellular concentrations of cholesterol and triglycerides by 40% and 20%, respectively. Puerarin stimulation significantly recovered cell viability and reduced cellular lipid accumulation to a level comparable to that in untreated control cells. Ethanol incubation reduced autophagy significantly as assessed by microtubule-associated protein 1 light chain 3 (LC3) expression using immunohistochemistry and immunoblot analysis. The reduced expression of LC3 was restored by puerarin in a dose-dependent manner in ethanol-treated cells. The effect of puerarin on mammalian targets of rapamycin (mTOR), a key regulator of autophagy, was examined in ethanol-treated hepatocytes. Immunoblotting revealed that puerarin significantly induced the phosphorylation of 5'AMP-activated protein kinase (AMPK), thereby suppressing the mTOR target proteins S6 ribosomal protein and 4E-binding protein 1. These data suggest that puerarin restored the viability of cells and reduced lipid accumulation in ethanol-treated hepatocytes by activating autophagy via AMPK/mTOR-mediated signaling. (C) 2011 Elsevier Inc. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.subject | MOLECULAR-MECHANISM | - |
dc.subject | LIPID-METABOLISM | - |
dc.subject | ALCOHOL | - |
dc.subject | LIVER | - |
dc.subject | PATHWAY | - |
dc.title | Restoration of autophagy by puerarin in ethanol-treated hepatocytes via the activation of AMP-activated protein kinase | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Sung-Joon | - |
dc.identifier.doi | 10.1016/j.bbrc.2011.09.077 | - |
dc.identifier.scopusid | 2-s2.0-80054836991 | - |
dc.identifier.wosid | 000296404800016 | - |
dc.identifier.bibliographicCitation | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.414, no.2, pp.361 - 366 | - |
dc.relation.isPartOf | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.citation.title | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.citation.volume | 414 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 361 | - |
dc.citation.endPage | 366 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.subject.keywordPlus | MOLECULAR-MECHANISM | - |
dc.subject.keywordPlus | LIPID-METABOLISM | - |
dc.subject.keywordPlus | ALCOHOL | - |
dc.subject.keywordPlus | LIVER | - |
dc.subject.keywordPlus | PATHWAY | - |
dc.subject.keywordAuthor | Puerarin | - |
dc.subject.keywordAuthor | Autophagy | - |
dc.subject.keywordAuthor | AMPK | - |
dc.subject.keywordAuthor | mTOR | - |
dc.subject.keywordAuthor | Alcoholic liver damage | - |
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