Production of 1,2-Propanediol from Glycerol in Saccharomyces cerevisiae
DC Field | Value | Language |
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dc.contributor.author | Jung, Joon-Young | - |
dc.contributor.author | Yun, Hyun Shik | - |
dc.contributor.author | Lee, Jinwon | - |
dc.contributor.author | Oh, Min-Kyu | - |
dc.date.accessioned | 2021-09-07T09:48:00Z | - |
dc.date.available | 2021-09-07T09:48:00Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2011-08 | - |
dc.identifier.issn | 1017-7825 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/111855 | - |
dc.description.abstract | Glycerol has become an attractive carbon source in the biotechnology industry owing to its low price and reduced state. However, glycerol is rarely used as a carbon source in Saccharomyces cerevisiae because of its low utilization rate. In this study, we used glycerol as a main carbon source in S. cerevisiae to produce 1,2-propanediol. Metabolically engineered S. cerevisiae strains with overexpression of glycerol dissimilation pathway genes, including glycerol kinase (GUT1), glycerol 3-phosphate dehydrogenase (GUT2), glycerol dehydrogenase (gdh), and a glycerol transporter gene (GUP1), showed increased glycerol utilization and growth rate. More significant improvement of glycerol utilization and growth rate was accomplished by introducing 1,2-propanediol pathway genes, mgs (methylglyoxal synthase) and gldA (glycerol dehydrogenase) from Escherichia coli. By engineering both glycerol dissimilation and 1,2-propanediol pathways, the glycerol utilization and growth rate were improved 141% and 77%, respectively, and a 2.19 g 1,2-propanediol/l titer was achieved in 1% (v/v) glycerol-containing YEPD medium in engineered S. cerevisiae. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY | - |
dc.subject | ESCHERICHIA-COLI | - |
dc.subject | ANAEROBIC FERMENTATION | - |
dc.subject | KLEBSIELLA-PNEUMONIAE | - |
dc.subject | ENHANCED PRODUCTION | - |
dc.subject | ENERGY-PRODUCTION | - |
dc.subject | DISSIMILATION | - |
dc.subject | ETHANOL | - |
dc.subject | YEAST | - |
dc.subject | PATHWAY | - |
dc.subject | GENE | - |
dc.title | Production of 1,2-Propanediol from Glycerol in Saccharomyces cerevisiae | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Oh, Min-Kyu | - |
dc.identifier.doi | 10.4014/jmb.1103.03009 | - |
dc.identifier.scopusid | 2-s2.0-80052280696 | - |
dc.identifier.wosid | 000294382300010 | - |
dc.identifier.bibliographicCitation | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.21, no.8, pp.846 - 853 | - |
dc.relation.isPartOf | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.citation.title | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.citation.volume | 21 | - |
dc.citation.number | 8 | - |
dc.citation.startPage | 846 | - |
dc.citation.endPage | 853 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART001578260 | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | ANAEROBIC FERMENTATION | - |
dc.subject.keywordPlus | KLEBSIELLA-PNEUMONIAE | - |
dc.subject.keywordPlus | ENHANCED PRODUCTION | - |
dc.subject.keywordPlus | ENERGY-PRODUCTION | - |
dc.subject.keywordPlus | DISSIMILATION | - |
dc.subject.keywordPlus | ETHANOL | - |
dc.subject.keywordPlus | YEAST | - |
dc.subject.keywordPlus | PATHWAY | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordAuthor | Saccharomyces cerevisiae | - |
dc.subject.keywordAuthor | glycerol | - |
dc.subject.keywordAuthor | 1,2-propanediol | - |
dc.subject.keywordAuthor | metabolic engineering | - |
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