Production of 1,2-Propanediol from Glycerol in Saccharomyces cerevisiae
- Authors
- Jung, Joon-Young; Yun, Hyun Shik; Lee, Jinwon; Oh, Min-Kyu
- Issue Date
- 8월-2011
- Publisher
- KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
- Keywords
- Saccharomyces cerevisiae; glycerol; 1,2-propanediol; metabolic engineering
- Citation
- JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.21, no.8, pp.846 - 853
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
- Volume
- 21
- Number
- 8
- Start Page
- 846
- End Page
- 853
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/111855
- DOI
- 10.4014/jmb.1103.03009
- ISSN
- 1017-7825
- Abstract
- Glycerol has become an attractive carbon source in the biotechnology industry owing to its low price and reduced state. However, glycerol is rarely used as a carbon source in Saccharomyces cerevisiae because of its low utilization rate. In this study, we used glycerol as a main carbon source in S. cerevisiae to produce 1,2-propanediol. Metabolically engineered S. cerevisiae strains with overexpression of glycerol dissimilation pathway genes, including glycerol kinase (GUT1), glycerol 3-phosphate dehydrogenase (GUT2), glycerol dehydrogenase (gdh), and a glycerol transporter gene (GUP1), showed increased glycerol utilization and growth rate. More significant improvement of glycerol utilization and growth rate was accomplished by introducing 1,2-propanediol pathway genes, mgs (methylglyoxal synthase) and gldA (glycerol dehydrogenase) from Escherichia coli. By engineering both glycerol dissimilation and 1,2-propanediol pathways, the glycerol utilization and growth rate were improved 141% and 77%, respectively, and a 2.19 g 1,2-propanediol/l titer was achieved in 1% (v/v) glycerol-containing YEPD medium in engineered S. cerevisiae.
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