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Quantitative analysis of specific target DNA oligomers using a DNA-immobilized packed-column system

Authors
Pack, Seung PilHeo, Tae-HweDevarayapalli, Kamakshaiah CharyuluMakino, Keisuke
Issue Date
8월-2011
Publisher
SPRINGER HEIDELBERG
Keywords
Bioanalytical methods; Chromatography; DNA-immobilized packed column; DNA oligomer separation
Citation
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, v.401, no.2, pp.667 - 676
Indexed
SCIE
SCOPUS
Journal Title
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume
401
Number
2
Start Page
667
End Page
676
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/111948
DOI
10.1007/s00216-011-5129-6
ISSN
1618-2642
Abstract
Although a DNA-immobilized packed-column (DNA-packed column), which relies on sequence-dependent interactions of target DNA or mRNA (in the mobile phase) with DNA probes (on the silica particle) in a continuous flow process, could be considered as an alternative platform for quantitative analysis of specific DNA to DNA chip methodology, the performance in practice has not been satisfactory. In this study, we set up a more efficient quantitative analysis system based on a DNA-packed column by employing a temperature-gradient strategy and DMSO-containing mobile phase. Using a temperature-gradient strategy based on T (m) values of probe/target DNA hybridizations and DMSO (5%)-containing mobile phase, we succeeded in the quantitative analysis of a specific complementary target distinguishable from non-complementary DNA oligomers or other similar DNA samples. In addition, two different target DNA oligomers even with similar T (m) values were separated and detected quantitatively by using a packed column carrying two different DNA probes.
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