GSK-3 beta-induced ASK1 stabilization is crucial in LPS-induced endotoxin shock
- Authors
- Noh, Kyung Tae; Park, Yeong-Min; Cho, Ssang-Goo; Choi, Eui-Ju
- Issue Date
- 15-7월-2011
- Publisher
- ELSEVIER INC
- Keywords
- ASK1; GSK-3 beta; p38; Stability; Endotoxemia
- Citation
- EXPERIMENTAL CELL RESEARCH, v.317, no.12, pp.1663 - 1668
- Indexed
- SCIE
SCOPUS
- Journal Title
- EXPERIMENTAL CELL RESEARCH
- Volume
- 317
- Number
- 12
- Start Page
- 1663
- End Page
- 1668
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/111981
- DOI
- 10.1016/j.yexcr.2011.03.022
- ISSN
- 0014-4827
- Abstract
- Glycogen synthase kinase-3 beta (GSK-3 beta), a multifunctional kinase, is a regulator of lipopolysaccharide (LPS)-mediated septic shock. Apoptosis signal-regulating kinase 1 (ASK1) is also required for LPS-induced activation of p38, which is a crucial determinant for the production of pro-inflammatory cytokines via Toll-like receptor 4 (TLR4) in endotoxemia. Here, we show that attenuation of endotoxemia induced by GSK-3 inhibition is caused by the ASK1 reduction-mediated inhibition of p38, a representative downstream kinase of ASK1. LPS-stimulated activation of p38 was blocked by the reduction of ASK1 via the knockdown of GSK-3 beta. In addition, compared with L929 control cells, ASK1 protein was reduced in L929 cells stably expressing Wnt-3a and in which beta-catenin was active, due to the inhibition of GSK-3 beta activity. GSK-3 beta inhibition-mediated ASK1 reduction was also confirmed by reduced ASK1 in GSK-3 beta-deficient mouse embryo fibroblasts (MEFs) and MCF7 GSK-3 beta siRNA cells. Furthermore, ASK1 protein stability was also attenuated in MCF7 GSK-3 beta siRNA cells compared with GFP control cells. Consistent with stability data, a much stronger ubiquitination of ASK1 was observed in cells in which GSK-3 beta was knocked down. These findings suggest that GSK-3 beta crosstalks with p38 kinase via the regulation of ASK1 protein stability in endotoxemia. (c) 2011 Elsevier Inc. All rights reserved.
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Collections - Graduate School > Department of Life Sciences > 1. Journal Articles
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