Comparison of Proteomes between Wheat-Rye Translocations and Their Recurrent Parents
- Authors
- Lee, T. G.; Johnson, J. W.; Seo, Y. W.
- Issue Date
- Jul-2011
- Publisher
- PLEIADES PUBLISHING INC
- Keywords
- 2BS.2RL wheat-rye translocationsHessian fly; proteome analysis
- Citation
- RUSSIAN JOURNAL OF PLANT PHYSIOLOGY, v.58, no.4, pp.703 - 714
- Indexed
- SCIE
SCOPUS
- Journal Title
- RUSSIAN JOURNAL OF PLANT PHYSIOLOGY
- Volume
- 58
- Number
- 4
- Start Page
- 703
- End Page
- 714
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/112079
- DOI
- 10.1134/S1021443711030095
- ISSN
- 1021-4437
- Abstract
- 2BS.2RL wheat-rye translocations have presented phenotypes of improved agronomic performance, such as Hessian fly resistance. The main objective of this work was to use two-dimensional electrophoresis to identify the proteomic differences between 2BS.2RL wheat-rye translocations and their recurrent parents. The investigation of seeds revealed line-specific protein spots, such as alpha-amylase inhibitor 0.19. More diverse expression patterns were observed in leaves than in seeds. Protein spots found specifically in 2BS.2RL, but not in non-2RL translocations were identified as beta-glucosidase and vacuolar ATP synthase subunit B2, demonstrating the effects of translocated rye chromatin 2RL on common wheat genetic back-ground. When the leaf protein spots were compared in the control and Hessian fly-infested near-isogenic line (NIL) (2BS.2RL), many down-regulated proteins and specific proteins, such as beta-glucosidase, were detected in the latter.
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Collections - Graduate School > Department of Plant Biotechnology > 1. Journal Articles
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