Effects of vibration on the proteome expression of anterior cruciate ligament cells
- Authors
- Han, Gi-Yeon; Park, Seung-Ah; Kim, Ji-Hye; Lee, Eun-Kyung; Kim, Hyun-Jung; Seo, Young-Kwon; Park, Jung-Keug; Kim, Chan-Wha
- Issue Date
- 7월-2011
- Publisher
- SAGE PUBLICATIONS LTD
- Keywords
- anterior cruciate ligament; extracellular matrix; vibration; annexin A2; prolyl 4 hydroxylase
- Citation
- EXPERIMENTAL BIOLOGY AND MEDICINE, v.236, no.7, pp.783 - 789
- Indexed
- SCIE
SCOPUS
- Journal Title
- EXPERIMENTAL BIOLOGY AND MEDICINE
- Volume
- 236
- Number
- 7
- Start Page
- 783
- End Page
- 789
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/112117
- DOI
- 10.1258/ebm.2011.010358
- ISSN
- 1535-3702
- Abstract
- Recent reports have suggested that vibration has beneficial effects on knee healing response; however, the biomechanism of these beneficial effects still need to be determined on the anterior cruciate ligament (ACL) cell level. In this study, we applied a 20 Hz vibration to ACL cells, which produced a 20% increase (P < 0.001) in cell activity and 17% increase (P < 0.001) in intracellular sulfated glycosaminoglycan levels. In the 20 Hz vibration-stimulated ACL cell group, eight up-regulated (100 similar to 300%) protein spots were identified compared with the control group by proteomics analysis. Among these proteins, Annexin A2 and Prolyl 4 hydroxylase (PH4B) were shown to have a 71% and 16% higher expression, respectively, in the 20 Hz vibration-stimulated ACL cell group by Western blotting (P < 0.001). These results indicate that vibration produces a positive cellular environment, and Annexin A2 and prolyl 4 hydroxylase are expected to help ligament repair and ACL cell proliferation by controlling cell membrane and extracellular matrix formation.
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Collections - College of Life Sciences and Biotechnology > Division of Life Sciences > 1. Journal Articles
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