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Miniaturized enzyme production and development of micro-assays for cellulolytic and xylanolytic enzymes
- Authors
- Lee, Young Min; Lee, Hanbyul; Kim, Gyu-Hyeok; Kim, Jae-Jin
- Issue Date
- 7월-2011
- Publisher
- ELSEVIER SCIENCE BV
- Keywords
- Cellulase; Enzyme assay; Enzyme production; Miniaturization; Xylanase
- Citation
- JOURNAL OF MICROBIOLOGICAL METHODS, v.86, no.1, pp.124 - 127
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF MICROBIOLOGICAL METHODS
- Volume
- 86
- Number
- 1
- Start Page
- 124
- End Page
- 127
- ISSN
- 0167-7012
- Abstract
- Miniaturized fungal cultivation and enzyme assays were developed. Cultivation for enzyme production was performed in 50 mL conical tubes. In addition, the miniaturized enzyme assays reduced the amount of enzymes and reagents necessary. These procedures can be adopted in screening fungi to determine if they produce cellulolytic and xylanolytic enzymes. (C) 2011 Elsevier B.V. All rights reserved.
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