Barley Intake Induces Bile Acid Excretion by Reduced Expression of Intestinal ASBT and NPC1L1 in C57BL/6J Mice
- Authors
- Hoang, Minh-Hien; Houng, Soung-Jin; Jun, Hee-Jin; Lee, Ji-Hae; Choi, Jin-Woong; Kim, So-Hee; Kim, Yong-Ro; Lee, Sung-Joon
- Issue Date
- 22-6월-2011
- Publisher
- AMER CHEMICAL SOC
- Keywords
- barley; cholesterol; bile acid; ASBT; NPC1L1
- Citation
- JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, v.59, no.12, pp.6798 - 6805
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
- Volume
- 59
- Number
- 12
- Start Page
- 6798
- End Page
- 6805
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/112212
- DOI
- 10.1021/jf200681n
- ISSN
- 0021-8561
- Abstract
- To investigate the hypocholesterolemic mechanism of barley in vivo, six-week-old C57BL/6J mice were fed a high-fat diet (HFD) or high-fat diet containing barley (HFD-B) for seven weeks. Total and LDL cholesterol concentrations were significantly reduced in the HFD-B group while fecal cholesterol and bile acid was increased. Real-time PCR and immunoblot analysis revealed the induction of FXR expression, which in turn suppressed the expression of ASBT and NPC1L1 in the HFD-B group compared with the controls. In the liver, the expression of HMG-CoA reductase was significantly reduced while LDL receptor expression was unaltered in the HFD-B group compared with the controls. Our data suggest that the hypocholesterolemic effects of barley are primarily the result of reduced dietary cholesterol uptake and bile acid resorption. Reduced expression of intestinal ASBT and NPC1L1 may play a key role in the regulation of dietary cholesterol and bile acid metabolism in mice consuming a diet containing barley.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - Graduate School > Department of Biotechnology > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.