Lipid-Soluble Ginseng Extract Induces Apoptosis and G0/G1 Cell Cycle Arrest in NCI-H460 Human Lung Cancer Cells
- Authors
- Kang, Moo Rim; Kim, Hwan Mook; Kang, Jong Soon; Lee, Kiho; Lee, Sung Dong; Hyun, Dong-Hoon; In, Man-Jin; Park, Song-Kyu; Kim, Dong Chung
- Issue Date
- 6월-2011
- Publisher
- SPRINGER
- Keywords
- Apoptosis induction; Caspase activation; Human lung cancer cell; G0/G1 phase arrest; Lipid-soluble ginseng extract
- Citation
- PLANT FOODS FOR HUMAN NUTRITION, v.66, no.2, pp.101 - 106
- Indexed
- SCIE
SCOPUS
- Journal Title
- PLANT FOODS FOR HUMAN NUTRITION
- Volume
- 66
- Number
- 2
- Start Page
- 101
- End Page
- 106
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/112397
- DOI
- 10.1007/s11130-011-0232-6
- ISSN
- 0921-9668
- Abstract
- This study was performed to elucidate the anticancer mechanism of a lipid-soluble ginseng extract (LSGE) by analyzing induction of apoptosis and arrest of cell cycle progression using the NCI-H460 human lung cancer cell line. Proliferation of NCI-H460 cells was potently inhibited by LSGE in a dose-dependent manner. The cell cycle arrest at the G0/G1 phase in NCI-H460 cells was induced by LSGE. The percentage of G0/G1 phase cells significantly increased, while that of S phase cells decreased after treatment with LSGE. The expression levels of cyclin-dependent kinase2 (CDK2), CDK4, CDK6, cyclin D3 and cyclin E related to G0/G1 cells progression were also altered by LSGE. In addition, LSGE-induced cell death occurred through apoptosis, which was accompanied by increasing the activity of caspases including caspase-8, caspase-9 and caspase-3. Consistent with enhancement of caspase activity, LSGE increased protein levels of cleaved caspase-3, caspase-8, caspase-9, and poly-ADP-ribose polymerase (PARP). These apoptotic effects of LSGE were inhibited by the pan-caspase inhibitor Z-VAD-fmk. These findings indicate that LSGE inhibits NCI-H460 human lung cancer cell growth by cell cycle arrest at the G0/G1 phase and induction of caspase-mediated apoptosis.
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