Aptamer Biosensors for Label-Free Colorimetric Detection of Human IgE Based on Polydiacetylene (PDA) Supramolecules
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Jun Pyo | - |
dc.contributor.author | Park, Cheol Hee | - |
dc.contributor.author | Sim, Sang Jun | - |
dc.date.accessioned | 2021-09-07T13:00:42Z | - |
dc.date.available | 2021-09-07T13:00:42Z | - |
dc.date.created | 2021-06-14 | - |
dc.date.issued | 2011-05 | - |
dc.identifier.issn | 1533-4880 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/112591 | - |
dc.description.abstract | In this study, we demonstrate an aptamer-based biosensor (apta-biosensor) using PDA liposomes for label-free detection of allergy diagnosis by hIgE detection. In order to detect the target hIgE, the surface of PDA liposome were functionalized with hIgE antibody and anti-hIgE aptamer as a receptor, and the target hIgE onto the receptors was detected by the change of fluorescence signal. The hIgE antibody-modified PDA liposome biosensor had a serious problem that the immune reaction between receptor and target could not powerfully affect the change of florescence signal on PDA liposorne. In order to solve this problem, the anti-hIgE aptamer which was far smaller than whole antibody was introduced as the receptor for the PDA liposome system. An aptamer-based PDA liposome biosensor was able to measure a quantity of target protein with various concentrations and at this time the detection limit was 141 ng/mL of the hIgE concentration. These results enabled diagnosis of allergy disease by an aptamer-based PDA liposome biosensor because real allergic patients showed high concentration of hIgE in serum (greater than 290 ng/mL). Therefore, we suggest that aptamer-modified PDA supramolecules as promising candidates for development of label-free colorimetric biosensors. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | AMER SCIENTIFIC PUBLISHERS | - |
dc.subject | IN-VITRO SELECTION | - |
dc.subject | VESICLES | - |
dc.subject | CHROMISM | - |
dc.subject | COLOR | - |
dc.subject | BIND | - |
dc.title | Aptamer Biosensors for Label-Free Colorimetric Detection of Human IgE Based on Polydiacetylene (PDA) Supramolecules | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Sim, Sang Jun | - |
dc.identifier.doi | 10.1166/jnn.2011.3664 | - |
dc.identifier.wosid | 000290692400079 | - |
dc.identifier.bibliographicCitation | JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY, v.11, no.5, pp.4269 - 4274 | - |
dc.relation.isPartOf | JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY | - |
dc.citation.title | JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY | - |
dc.citation.volume | 11 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 4269 | - |
dc.citation.endPage | 4274 | - |
dc.type.rims | ART | - |
dc.type.docType | Article; Proceedings Paper | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalResearchArea | Physics | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Physics, Applied | - |
dc.relation.journalWebOfScienceCategory | Physics, Condensed Matter | - |
dc.subject.keywordPlus | IN-VITRO SELECTION | - |
dc.subject.keywordPlus | VESICLES | - |
dc.subject.keywordPlus | CHROMISM | - |
dc.subject.keywordPlus | COLOR | - |
dc.subject.keywordPlus | BIND | - |
dc.subject.keywordAuthor | Polydiacetylene | - |
dc.subject.keywordAuthor | Biosensor | - |
dc.subject.keywordAuthor | Aptamer | - |
dc.subject.keywordAuthor | IgE | - |
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