Aptamer Biosensors for Label-Free Colorimetric Detection of Human IgE Based on Polydiacetylene (PDA) Supramolecules
- Authors
- Kim, Jun Pyo; Park, Cheol Hee; Sim, Sang Jun
- Issue Date
- 5월-2011
- Publisher
- AMER SCIENTIFIC PUBLISHERS
- Keywords
- Polydiacetylene; Biosensor; Aptamer; IgE
- Citation
- JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY, v.11, no.5, pp.4269 - 4274
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
- Volume
- 11
- Number
- 5
- Start Page
- 4269
- End Page
- 4274
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/112591
- DOI
- 10.1166/jnn.2011.3664
- ISSN
- 1533-4880
- Abstract
- In this study, we demonstrate an aptamer-based biosensor (apta-biosensor) using PDA liposomes for label-free detection of allergy diagnosis by hIgE detection. In order to detect the target hIgE, the surface of PDA liposome were functionalized with hIgE antibody and anti-hIgE aptamer as a receptor, and the target hIgE onto the receptors was detected by the change of fluorescence signal. The hIgE antibody-modified PDA liposome biosensor had a serious problem that the immune reaction between receptor and target could not powerfully affect the change of florescence signal on PDA liposorne. In order to solve this problem, the anti-hIgE aptamer which was far smaller than whole antibody was introduced as the receptor for the PDA liposome system. An aptamer-based PDA liposome biosensor was able to measure a quantity of target protein with various concentrations and at this time the detection limit was 141 ng/mL of the hIgE concentration. These results enabled diagnosis of allergy disease by an aptamer-based PDA liposome biosensor because real allergic patients showed high concentration of hIgE in serum (greater than 290 ng/mL). Therefore, we suggest that aptamer-modified PDA supramolecules as promising candidates for development of label-free colorimetric biosensors.
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Collections - College of Engineering > Department of Chemical and Biological Engineering > 1. Journal Articles
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