Application of an enzyme-based biofuel cell containing a bioelectrode modified with deoxyribonucleic acid-wrapped single-walled carbon nanotubes to serum
- Authors
- Lee, Jin Young; Shin, Hyun Yong; Kang, Seong Woo; Park, Chulhwan; Kim, Seung Wool
- Issue Date
- 5-1월-2011
- Publisher
- ELSEVIER SCIENCE INC
- Keywords
- Enzyme-based biofuel cell; Glucose oxidase; Carbon nanotubes; Enzyme immobilization; Enzyme activity; Serum
- Citation
- ENZYME AND MICROBIAL TECHNOLOGY, v.48, no.1, pp.80 - 84
- Indexed
- SCIE
SCOPUS
- Journal Title
- ENZYME AND MICROBIAL TECHNOLOGY
- Volume
- 48
- Number
- 1
- Start Page
- 80
- End Page
- 84
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/113286
- DOI
- 10.1016/j.enzmictec.2010.09.010
- ISSN
- 0141-0229
- Abstract
- Enzyme-based biofuel cells (EFCs) are a form of biofuel cells (BFCs) that can utilize redox enzymes as biocatalysts. Applications of an EFC to an implantable system are evaluated under mild conditions, such as ambient temperature or neutral pH. In the present study, an EFC containing a bioelectrode modified with deoxyribonucleic acid (DNA)-wrapped single-walled carbon nanotubes (SWNTs) was applied to a serum system. The protection of immobilized glucose oxidase (GOD) using DNA-wrapped SWNTs was investigated in a trypsin environment, which can exist in a serum. GOD is immobilized by masking the active site onto the anode electrode. The anode/cathode system in the cell was composed of GOD/laccase as the biocatalysts and glucose/oxygen as the substrates in serum. The electrical properties of the anode in serum according to cyclic voltammetry (CV cycle) were improved using the DNA-wrapped SWNTs. Overall, an EFC that employed DNA-wrapped SWNTs and GOD immobilization in conjunction with protection of the active site increased the stability of GOD in serum, which enabled a high level of power production (ca. 190 mu W/cm(2)) for up to 1 week. (C) 2010 Elsevier Inc. All rights reserved.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Engineering > Department of Chemical and Biological Engineering > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.