Enterobacter aerogenes를 이용한 crude glycerol 기반의 바이오에탄올 생산

Abstract

The effects of pH, glycerol concentration and salt on cell growth and ethanol production using Enterobacter aerogenes KCTC 2190 were evaluated in the anaerobic culture condition. In condition of initial pH 5, cell growth and ethanol production were highest. An initial concentration of 10 g/L of pure glycerol gave the highest cell growth and ethanol production. However, in case of over 15 g/L of pure glycerol,they decreased. The cell growth and ethanol production decreased with the increase of salt concentration. When 10 g/L of crude glycerol was used as the carbon source, the cell growth and ethanol production were 1.32 OD600 and 3.95 g/L,respectively, which were about 94.4% and 88.5% compared to those of pure glycerol. These result indicates that the crude glycerol produced in the biodiesel manufacturing process may be useful as a potential carbon source for ethanol production form Enterobacter aerogenes KCTC 2190.