Effects of the immobilization of heparin and rhPDGF-BB to titanium surfaces for the enhancement of osteoblastic functions and anti-inflammation
- Authors
- Huh, Jung-Bo; Lee, Jeong-Yol; Lee, Kyung-Lae; Kim, Sung-Eun; Yun, Mi-Jung; Shim, Ji-Suk; Shim, June-Sung; Shin, Sang-Wan
- Issue Date
- 2011
- Publisher
- KOREAN ACAD PROSTHODONTICS
- Keywords
- Titanium; Heparin; rhPDGF-BB; Anti-inflammation; Osteoblastic function
- Citation
- JOURNAL OF ADVANCED PROSTHODONTICS, v.3, no.3, pp.152 - 160
- Indexed
- SCOPUS
KCI
- Journal Title
- JOURNAL OF ADVANCED PROSTHODONTICS
- Volume
- 3
- Number
- 3
- Start Page
- 152
- End Page
- 160
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/114971
- DOI
- 10.4047/jap.2011.3.3.152
- ISSN
- 2005-7806
- Abstract
- PURPOSE. This study was to investigate the effects of recombinant human platelet-derived growth factor (rhPDGF-BB) and heparin to titanium surfaces for enhancement of osteoblastic functions and inhibition of inflammation activity. MATERIALS AND METHODS. The anodized titanium discs, not coated with any material, were used as a control group. In heparinized- Ti group, dopamine was anchored to the surface of Ti substrates, and coated with heparin. In PDGF-Ti group, rhPDGF-BB was immobilized onto heparinized Ti surface. The surface morphologies were investigated by the scanning electron microscope in each group. The release kinetics of rhPDGF-BB were analyzed, and cytotoxicity tests for each group were conducted. The biocompatibilities were characterized by measuring cell proliferation, alkaline phosphatase activity, and calcium deposition using MG-63 cells. Statistical comparisons were carried out by one-way ANOVA tests. Differences were considered statistically significant at *P<.05 and **P<.001. RESULTS. The combination of rhPDGF-BB and heparin stimulated alkaline phosphatase activity and OCN mRNA expression in osteoblastic cells (*P<.05 and **P<.001). MG-63 cells grown on PDGF-Ti had significantly higher amounts of calcium deposition than those grown on anodized Ti (**P<.001). Heparinized Ti was more anti-inflammatory compared to anodized Ti, when exposed to lipopolysaccharide using the transcript levels of TNF-alpha and IL-6 of proinflammatory cytokine (*P<.05 and **P<.001) CONCLUSION. The result of this study demonstrated that the incorporation of rhPDGF-BB and heparin onto Ti surface enhanced osteoblastic functions and inhibited inflammation. [J Adv Prosthodont 2011;3:152-60]
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