Separation of platelets from whole blood using standing surface acoustic waves in a microchannel
- Authors
- Nam, Jeonghun; Lim, Hyunjung; Kim, Dookon; Shin, Sehyun
- Issue Date
- 2011
- Publisher
- ROYAL SOC CHEMISTRY
- Citation
- LAB ON A CHIP, v.11, no.19, pp.3361 - 3364
- Indexed
- SCIE
SCOPUS
- Journal Title
- LAB ON A CHIP
- Volume
- 11
- Number
- 19
- Start Page
- 3361
- End Page
- 3364
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/115048
- DOI
- 10.1039/c1lc20346k
- ISSN
- 1473-0197
- Abstract
- Platelet separation from blood is essential for biochemical analyses and clinical diagnosis. In this article, we propose a method to separate platelets from undiluted whole blood using standing surface acoustic waves (SSAWs) in a microfluidic device. A polydimethylsiloxane (PDMS) microfluidic channel was fabricated and integrated with interdigitated transducer (IDT) electrodes patterned on a piezoelectric substrate. To avoid shear-induced activation of platelets, the blood sample flow was hydrodynamically focused by introducing sheath flow from two side-inlets and pressure nodes were designed to locate at side walls. By means of flow cytometric analysis, the RBC clearance ratio from whole blood was found to be over 99% and the purity of platelets was close to 98%. Conclusively, the present technique using SSAWs can directly separate platelets from undiluted whole blood with higher purity than other methods.
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Collections - College of Engineering > Department of Mechanical Engineering > 1. Journal Articles
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