Detailed Information

Cited 0 time in webofscience Cited 0 time in scopus
Metadata Downloads

Robust Trypsin Coating on Electrospun Polymer Nanofibers in Rigorous Conditions and Its Uses for Protein Digestion

Authors
Ahn, Hye-KyungKim, Byoung ChanJun, Seung-HyunChang, Mun SeockLopez-Ferrer, DanielSmith, Richard D.Gu, Man BockLee, Sang-WonKim, Beom SooKim, Jungbae
Issue Date
15-Dec-2010
Publisher
WILEY
Keywords
enzyme coating; trypsin; electrospun nanofibers; protein digestion
Citation
BIOTECHNOLOGY AND BIOENGINEERING, v.107, no.6, pp.917 - 923
Indexed
SCIE
SCOPUS
Journal Title
BIOTECHNOLOGY AND BIOENGINEERING
Volume
107
Number
6
Start Page
917
End Page
923
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/115115
DOI
10.1002/bit.22895
ISSN
0006-3592
Abstract
An efficient protein digestion in proteomic analysis requires the stabilization of proteases such as trypsin. In the present work, trypsin was stabilized in the form of enzyme coating on electrospun polymer nanofibers (EC-TR), which crosslinks additional trypsin molecules onto covalently attached trypsin (CA-TR). EC-TR showed better stability than CA-TR in rigorous conditions, such as at high temperatures of 40 and 50 degrees C, in the presence of organic co-solvents, and at various pH's. For example, the half-lives of CA-TR and EC-TR were 1.42 and 231 h at 40 degrees C, respectively. The improved stability of EC-TR can be explained by covalent linkages on the surface of trypsin molecules, which effectively inhibits the denaturation, autolysis, and leaching of trypsin. The protein digestion was performed at 40 degrees C by using both CA-TR and EC-TR in digesting a model protein, enolase. EC-TR showed better performance and stability than CA-TR by maintaining good performance of enolase digestion under recycled uses for a period of 1 week. In the same condition, CA-TR showed poor performance from the beginning and could not be used for digestion at all after a few usages. The enzyme coating approach is anticipated to be successfully employed not only for protein digestion in proteomic analysis but also for various other fields where the poor enzyme stability presently hampers the practical applications of enzymes. Biotechnol. Bioeng. 2010;107: 917-923. (C) 2010 Wiley Periodicals, Inc.
Files in This Item
There are no files associated with this item.
Appears in
Collections
Graduate School > Department of Biotechnology > 1. Journal Articles
College of Science > Department of Chemistry > 1. Journal Articles
College of Engineering > Department of Chemical and Biological Engineering > 1. Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher Kim, Jung bae photo

Kim, Jung bae
College of Engineering (Department of Chemical and Biological Engineering)
Read more

Altmetrics

Total Views & Downloads

BROWSE