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Quantitative Detection of Glyphosate by Simultaneous Analysis of UV Spectroscopy and Fluorescence Using DNA-Labeled Gold Nanoparticles
- Authors
- Lee, Hee Uk; Shin, Hyun Yong; Lee, Jin Young; Song, Yoon Seok; Park, ChulHwan; Kim, Seung Wook
- Issue Date
- 8-12월-2010
- Publisher
- AMER CHEMICAL SOC
- Keywords
- Immunosensor; gold nanoparticles; antigen-antibody; glyphosate; fluorescence; quantitative analysis
- Citation
- JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, v.58, no.23, pp.12096 - 12100
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
- Volume
- 58
- Number
- 23
- Start Page
- 12096
- End Page
- 12100
- ISSN
- 0021-8561
- Abstract
- A sandwich-type immunosensor composed of antigen double target/probe DNA-coated gold nanoparticles (NPs) was developed for the measurement of fluorescence intensity and quantitative analysis of single-stranded DNA based on the concentration of free glyphosate. The reaction between the antigen double DNA gold NPs and immobilized antibody on the substrate was carried out for 2 h. The results of the antigen antibody reaction were measured on the basis of the fluorescence intensity obtained from comparison with the free antigens at concentrations of 0.01-100 mu g mL(-1) for the detection of immobilized antigen-double DNA-gold NPs. For the quantitative analysis based on the concentration of glyphosate(0.01-100 mu g mL(-1)), the immunosensor response also revealed the same detection range of glyphosate using DNA detection.
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