A Three-Dimensional Nanostructured Array of Protein Nanoparticles
- Authors
- Seo, Hyuk-Seong; Kim, Seong-Eun; Park, Jin-Seung; Lee, Jong-Hwan; Yang, Ki-Yeon; Lee, Heon; Lee, Kyung Eun; Han, Sung-Sik; Lee, Jeewon
- Issue Date
- 8-12월-2010
- Publisher
- WILEY-V C H VERLAG GMBH
- Keywords
- biosensors; nanoparticles; nanostructures; protein engineering
- Citation
- ADVANCED FUNCTIONAL MATERIALS, v.20, no.23, pp.4055 - 4061
- Indexed
- SCIE
SCOPUS
- Journal Title
- ADVANCED FUNCTIONAL MATERIALS
- Volume
- 20
- Number
- 23
- Start Page
- 4055
- End Page
- 4061
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/115125
- DOI
- 10.1002/adfm.201001144
- ISSN
- 1616-301X
- Abstract
- Here a novel technique is reported to construct a three-dimensional (3D) array of well-defined and controllable multilayered nanostructures of proteins that is based on alternate layer-by-layer assembly of bacterial protein nanoparticles and DNA on a patterned array of gold dots. This is the first report on protein-based multilayer stacking, which has the following significant advantages over conventional multilayer assemblies: 1) avoiding hazardous chemicals, the multilayer assembly is implemented in aqueous solution under mild temperature and pH conditions over a relatively short period; 2) direct multilayer growth from designated position is possible by controlling the aspect ratio; 3) multicomponent stacking can be easily performed through alternate stacking of different building blocks (in this case protein nanoparticles); and 4) a wide variety of 3D arrays can be constructed using various functionalized protein nanoparticles that are easily prepared through a simple genetic engineering approach. In this study, as a proof of concept, the developed 3D and patterned arrays of protein nanoparticle multilayers are successfully applied to the multiplexed bioassays of breast and colorectal cancer markers.
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Collections - College of Engineering > Department of Chemical and Biological Engineering > 1. Journal Articles
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