Detailed Information

Cited 0 time in webofscience Cited 0 time in scopus
Metadata Downloads

Cloning and characterization of the new multiple stress responsible gene I (MuSI) from sweet potato

Authors
Seo, Sang-GyuKim, Ji-SeoungYang, Yu-SunJun, Byung-KiKang, Seung-WonLee, Gung-PyoKim, WookKim, Jong-BoLee, Hyeong-UnKim, Sun-Hyung
Issue Date
12월-2010
Publisher
SPRINGER
Keywords
Ipomoea batatas (L.) Lam.; MuSI; REF domain; Stress response
Citation
GENES & GENOMICS, v.32, no.6, pp.544 - 552
Indexed
SCIE
SCOPUS
KCI
Journal Title
GENES & GENOMICS
Volume
32
Number
6
Start Page
544
End Page
552
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/115251
DOI
10.1007/s13258-010-0093-7
ISSN
1976-9571
Abstract
New multiple-stress related gene isolated from sweet potato and designated it as MusI (multiple stress responsible gene I). Sequence analysis revealed that its full length cDNA was 998 bp long and included a 717 bp open reading frame encoding for 238 amino acids. Comparison of its cDNA and genomic DNA sequence showed that 3 exons were divided by 2 introns in its ORF region. Its deduced amino acid sequence contained a conserved rubber elongation factor (REF) domain and showed high homology with many stress-related proteins. Therefore, it was named MuSI (multiple stress responsible gene I). Southern hybridization analysis indicated that the MuSI gene may belong to a multi-gene family. Expression pattern of the MuSI gene showed that it was differently expressed among roots, stems, leaves, and flowers of a sweet potato, and its expression level was especially high in flowers and white fibrous roots. Its expression was also highly induced by various stress signals including dehydration, high salt, heavy metal, oxidation, and plant hormones. Stress tolerance experiment using transgenic plants overexpressing the MuSI gene showed that all independent transgenic tobacco lines have enhanced tolerance to high temperature stress. Among them, transgenic line 6 particularly showed tolerance to salt, heavy metal, and osmotic stress as well. These results suggest that the MuSI gene functions as a positive regulator of various stress responses and may be useful in improving stress tolerance of transgenic plants.
Files in This Item
There are no files associated with this item.
Appears in
Collections
Graduate School > Department of Plant Biotechnology > 1. Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher Kim, Wook photo

Kim, Wook
식물생명공학과
Read more

Altmetrics

Total Views & Downloads

BROWSE