Phenotypic and physiological alterations by heterologous acylhomoserine lactone synthase expression in Pseudomonas putida
- Authors
- Lee, Yunho; Yeom, Jinki; Kim, Jisun; Jung, Jaejoon; Jeon, Che Ok; Park, Woojun
- Issue Date
- 12월-2010
- Publisher
- MICROBIOLOGY SOC
- Citation
- MICROBIOLOGY-SGM, v.156, pp.3762 - 3772
- Indexed
- SCIE
SCOPUS
- Journal Title
- MICROBIOLOGY-SGM
- Volume
- 156
- Start Page
- 3762
- End Page
- 3772
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/115293
- DOI
- 10.1099/mic.0.041095-0
- ISSN
- 1350-0872
- Abstract
- Many bacteria harbour an incomplete quorum-sensing (OS) system, whereby they possess LuxR homologues without the OS acylhomoserine lactone (AHL) synthase, which is encoded by a luxl homologue. An artificial AHL-producing plasmid was constructed using a cvil gene encoding the C6-AHL [N-hexanoyl homoserine lactone (HHL)] synthase from Chromobacterium violaceum, and was introduced successfully into both the wild-type and a ppoR (luxR homologue) mutant of Pseudomonas putida. Our data provide evidence to suggest that the PpoR-HHL complex, but neither PpoR nor HHL alone, could attenuate growth, antibiotic resistance and biofilm formation ability. In contrast, swimming motility, siderophore production and indole degradation were enhanced by PpoR-HHL. The addition of exogenous indole increased biofilm formation and reduced swimming motility. Interestingly, indole proved ineffective in the presence of PpoR-HHL, thereby suggesting that the PpoR-HHL complex masks the effects of indole. Our data were supported by transcriptome analyses, which showed that the presence of the plasmid-encoded AHL synthase altered the expression of many genes on the chromosome in strain KT2440. Our results showed that heterologous luxl expression that occurs via horizontal gene transfer can regulate a broad range specific target genes, resulting in alterations of the phenotype and physiology of host cells.
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Collections - College of Life Sciences and Biotechnology > Division of Environmental Science and Ecological Engineering > 1. Journal Articles
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