Unidirectional signaling triggered through 2B4 (CD244), not CD48, in murine NK cells
DC Field | Value | Language |
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dc.contributor.author | Kim, Eun-Ok | - |
dc.contributor.author | Kim, Nayoung | - |
dc.contributor.author | Kim, Tae-Jin | - |
dc.contributor.author | Kim, Kwanghee | - |
dc.contributor.author | Kim, Tae Woo | - |
dc.contributor.author | Kumar, Vinay | - |
dc.contributor.author | Lee, Kyung-Mi | - |
dc.date.accessioned | 2021-09-07T23:43:43Z | - |
dc.date.available | 2021-09-07T23:43:43Z | - |
dc.date.created | 2021-06-14 | - |
dc.date.issued | 2010-10 | - |
dc.identifier.issn | 0741-5400 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/115567 | - |
dc.description.abstract | Engagement of 2B4 (CD244) with CD48 results in activation, costimulation, or inhibition of NK cell activities, depending on the cell types and the stage of differentiation. In vivo, 2B4(+) NK cells can interact with CD48(+) NK cells and also with surrounding CD48(+) hematopoietic cells. Similarly, CD48(+) NK cells may be triggered by adjacent 2B4(+) NK cells or other hematopoietic cells expressing 2B4, e. g., monocytes, basophils, gamma delta T cells, etc. As CD48 was also shown to function as an activating receptor, 2B4/CD48 binding in the settings of NK-to-NK or NK-to-non-NK cell interactions may generate bidirectional signals. To address this question, we examined the consequence of CD48 or 2B4 ligation using two experimental settings: one with target (syngeneic EL4 and allogeneic P815) cells, ectopically expressing surface 2B4 or CD48, and the other with direct cross-linking with plate-bound mAb. Here, we report that ligation of CD48 with 2B4(+) EL4 or 2B4(+) P815 targets, in the absence of other receptor engagement, did not alter NK cell cytotoxicity or proliferation significantly. Similarly, cross-linking of NK cells with plate-bound anti-CD48 mAb in the absence or presence of a suboptimal dose of IL-2 did not modulate NK proliferation, cytotoxicity, or cytokine production. Nonetheless, 2B4 cross-linking promoted NK cell proliferation and effector functions consistently in both settings. Therefore, our results demonstrate unequivocally that CD48 on surrounding NK or non-NK cells serves primarily as a ligand to stimulate 2B4 on the adjacent NK cells in mice. J. Leukoc. Biol. 88: 707-714; 2010. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | WILEY | - |
dc.subject | NATURAL-KILLER-CELLS | - |
dc.subject | 2B4/CD48 INTERACTIONS | - |
dc.subject | INHIBITORY RECEPTORS | - |
dc.subject | COUNTER-RECEPTOR | - |
dc.subject | MOLECULAR-BASIS | - |
dc.subject | CUTTING EDGE | - |
dc.subject | MOUSE CD2 | - |
dc.subject | IN-VITRO | - |
dc.subject | T-CELLS | - |
dc.subject | CYTOKINE | - |
dc.title | Unidirectional signaling triggered through 2B4 (CD244), not CD48, in murine NK cells | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Tae Woo | - |
dc.identifier.doi | 10.1189/jlb.0410198 | - |
dc.identifier.scopusid | 2-s2.0-77957868235 | - |
dc.identifier.wosid | 000285195400012 | - |
dc.identifier.bibliographicCitation | JOURNAL OF LEUKOCYTE BIOLOGY, v.88, no.4, pp.707 - 714 | - |
dc.relation.isPartOf | JOURNAL OF LEUKOCYTE BIOLOGY | - |
dc.citation.title | JOURNAL OF LEUKOCYTE BIOLOGY | - |
dc.citation.volume | 88 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 707 | - |
dc.citation.endPage | 714 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalResearchArea | Hematology | - |
dc.relation.journalResearchArea | Immunology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Hematology | - |
dc.relation.journalWebOfScienceCategory | Immunology | - |
dc.subject.keywordPlus | NATURAL-KILLER-CELLS | - |
dc.subject.keywordPlus | 2B4/CD48 INTERACTIONS | - |
dc.subject.keywordPlus | INHIBITORY RECEPTORS | - |
dc.subject.keywordPlus | COUNTER-RECEPTOR | - |
dc.subject.keywordPlus | MOLECULAR-BASIS | - |
dc.subject.keywordPlus | CUTTING EDGE | - |
dc.subject.keywordPlus | MOUSE CD2 | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | T-CELLS | - |
dc.subject.keywordPlus | CYTOKINE | - |
dc.subject.keywordAuthor | innate immunity | - |
dc.subject.keywordAuthor | NK activating receptors | - |
dc.subject.keywordAuthor | IFN-gamma | - |
dc.subject.keywordAuthor | natural cytotoxity | - |
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