Unidirectional signaling triggered through 2B4 (CD244), not CD48, in murine NK cells
- Authors
- Kim, Eun-Ok; Kim, Nayoung; Kim, Tae-Jin; Kim, Kwanghee; Kim, Tae Woo; Kumar, Vinay; Lee, Kyung-Mi
- Issue Date
- 10월-2010
- Publisher
- WILEY
- Keywords
- innate immunity; NK activating receptors; IFN-gamma; natural cytotoxity
- Citation
- JOURNAL OF LEUKOCYTE BIOLOGY, v.88, no.4, pp.707 - 714
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF LEUKOCYTE BIOLOGY
- Volume
- 88
- Number
- 4
- Start Page
- 707
- End Page
- 714
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/115567
- DOI
- 10.1189/jlb.0410198
- ISSN
- 0741-5400
- Abstract
- Engagement of 2B4 (CD244) with CD48 results in activation, costimulation, or inhibition of NK cell activities, depending on the cell types and the stage of differentiation. In vivo, 2B4(+) NK cells can interact with CD48(+) NK cells and also with surrounding CD48(+) hematopoietic cells. Similarly, CD48(+) NK cells may be triggered by adjacent 2B4(+) NK cells or other hematopoietic cells expressing 2B4, e. g., monocytes, basophils, gamma delta T cells, etc. As CD48 was also shown to function as an activating receptor, 2B4/CD48 binding in the settings of NK-to-NK or NK-to-non-NK cell interactions may generate bidirectional signals. To address this question, we examined the consequence of CD48 or 2B4 ligation using two experimental settings: one with target (syngeneic EL4 and allogeneic P815) cells, ectopically expressing surface 2B4 or CD48, and the other with direct cross-linking with plate-bound mAb. Here, we report that ligation of CD48 with 2B4(+) EL4 or 2B4(+) P815 targets, in the absence of other receptor engagement, did not alter NK cell cytotoxicity or proliferation significantly. Similarly, cross-linking of NK cells with plate-bound anti-CD48 mAb in the absence or presence of a suboptimal dose of IL-2 did not modulate NK proliferation, cytotoxicity, or cytokine production. Nonetheless, 2B4 cross-linking promoted NK cell proliferation and effector functions consistently in both settings. Therefore, our results demonstrate unequivocally that CD48 on surrounding NK or non-NK cells serves primarily as a ligand to stimulate 2B4 on the adjacent NK cells in mice. J. Leukoc. Biol. 88: 707-714; 2010.
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