Kinetic analysis of RNA interference for lamin A/C in HeLa cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | You, Sung-Ju | - |
dc.contributor.author | Lee, Sang-Hoon | - |
dc.contributor.author | Lee, Jae-Seung | - |
dc.date.accessioned | 2021-09-08T00:34:58Z | - |
dc.date.available | 2021-09-08T00:34:58Z | - |
dc.date.created | 2021-06-14 | - |
dc.date.issued | 2010-09 | - |
dc.identifier.issn | 1672-9145 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/115790 | - |
dc.description.abstract | We kinetically analyzed RNA interference (RNAi) for lamin A/C in HeLa cells, assuming suppression and recovery phases of gene expression, and dilution of transfected small interfering RNA (siRNA) by cell divisions. We observed the inhibitory effect of RNAi over a period of 6 days using various siRNA concentrations, and the maximum gene silencing efficiency occurred at Day 2 or 3. The gene silencing efficiency as a function of time and siRNA concentration was further quantitatively evaluated using a kinetic analysis method, demonstrating that RNAi for lamin A/C can be understood as a conventional drug-response system. This work provides potentially important guidelines for future applications using nanomaterials as delivery vehicles of siRNA in RNAi for lamin A/C. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | OXFORD UNIV PRESS | - |
dc.subject | INTRACELLULAR DELIVERY | - |
dc.subject | SIRNA DELIVERY | - |
dc.subject | GENE | - |
dc.subject | REPLICATION | - |
dc.subject | INHIBITION | - |
dc.subject | TIME | - |
dc.title | Kinetic analysis of RNA interference for lamin A/C in HeLa cells | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Sang-Hoon | - |
dc.contributor.affiliatedAuthor | Lee, Jae-Seung | - |
dc.identifier.doi | 10.1093/abbs/gmq068 | - |
dc.identifier.scopusid | 2-s2.0-77956271412 | - |
dc.identifier.wosid | 000281323400004 | - |
dc.identifier.bibliographicCitation | ACTA BIOCHIMICA ET BIOPHYSICA SINICA, v.42, no.9, pp.623 - 627 | - |
dc.relation.isPartOf | ACTA BIOCHIMICA ET BIOPHYSICA SINICA | - |
dc.citation.title | ACTA BIOCHIMICA ET BIOPHYSICA SINICA | - |
dc.citation.volume | 42 | - |
dc.citation.number | 9 | - |
dc.citation.startPage | 623 | - |
dc.citation.endPage | 627 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.subject.keywordPlus | INTRACELLULAR DELIVERY | - |
dc.subject.keywordPlus | SIRNA DELIVERY | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordPlus | REPLICATION | - |
dc.subject.keywordPlus | INHIBITION | - |
dc.subject.keywordPlus | TIME | - |
dc.subject.keywordAuthor | RNAi | - |
dc.subject.keywordAuthor | siRNA | - |
dc.subject.keywordAuthor | lamin A | - |
dc.subject.keywordAuthor | C | - |
dc.subject.keywordAuthor | kinetic analysis | - |
dc.subject.keywordAuthor | gene silencing | - |
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