Kinetic analysis of RNA interference for lamin A/C in HeLa cells
- Authors
- You, Sung-Ju; Lee, Sang-Hoon; Lee, Jae-Seung
- Issue Date
- 9월-2010
- Publisher
- OXFORD UNIV PRESS
- Keywords
- RNAi; siRNA; lamin A; C; kinetic analysis; gene silencing
- Citation
- ACTA BIOCHIMICA ET BIOPHYSICA SINICA, v.42, no.9, pp.623 - 627
- Indexed
- SCIE
SCOPUS
- Journal Title
- ACTA BIOCHIMICA ET BIOPHYSICA SINICA
- Volume
- 42
- Number
- 9
- Start Page
- 623
- End Page
- 627
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/115790
- DOI
- 10.1093/abbs/gmq068
- ISSN
- 1672-9145
- Abstract
- We kinetically analyzed RNA interference (RNAi) for lamin A/C in HeLa cells, assuming suppression and recovery phases of gene expression, and dilution of transfected small interfering RNA (siRNA) by cell divisions. We observed the inhibitory effect of RNAi over a period of 6 days using various siRNA concentrations, and the maximum gene silencing efficiency occurred at Day 2 or 3. The gene silencing efficiency as a function of time and siRNA concentration was further quantitatively evaluated using a kinetic analysis method, demonstrating that RNAi for lamin A/C can be understood as a conventional drug-response system. This work provides potentially important guidelines for future applications using nanomaterials as delivery vehicles of siRNA in RNAi for lamin A/C.
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Collections - College of Health Sciences > School of Biomedical Engineering > 1. Journal Articles
- College of Engineering > Department of Materials Science and Engineering > 1. Journal Articles
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