Class II beta-tubulin is a novel marker for human tonsillar M cells and follicular dendritic cells
- Authors
- Lee, Ju-Han; Kong, Su-Kang; Wu, Zheng-Sheng; Wu, Qiang; Choe, Jongseon; Cho, Hyun Yee; Ha, Seung Yeon; Kee, Sun Ho; Kim, Young-Sik
- Issue Date
- 8월-2010
- Publisher
- WILEY-BLACKWELL
- Keywords
- follicular dendritic cell; M cell; palatine tonsil; tubulin
- Citation
- JOURNAL OF ORAL PATHOLOGY & MEDICINE, v.39, no.7, pp.533 - 539
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF ORAL PATHOLOGY & MEDICINE
- Volume
- 39
- Number
- 7
- Start Page
- 533
- End Page
- 539
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/115963
- DOI
- 10.1111/j.1600-0714.2009.00865.x
- ISSN
- 0904-2512
- Abstract
- Objective: Membranous (M) cell of the human palatine tonsil is an antigen entry site for mucosal infection, but its location is obscure in histological sections. Recently, a microarray analysis has demonstrated that clusterin, annexin A5, CD44, MMP14, and beta-tubulin are candidate genes of M cell marker in mice. Among these genes, we here describe class II beta-tubulin as a new marker for human tonsillar M cells and follicular dendritic cells (FDCs), and present its usefulness for diagnosis of angioimmunoblastic T-cell lymphomas (AILTs). Materials and methods: Immunohistochemistry and Western blotting for class II beta-tubulin were performed using 81 cases of lymphoid, gastrointestinal and thyroid tissues, and an FDC cell line, respectively. Double immunostaining with clusterin and class II beta-tubulin were carried out. Results: Class II beta-tubulin localized the M cells and FDCs in the palatine tonsils (10/10, 100%) and adenoids (10/10, 100%). It was colocalized with clusterin in the palatine tonsils. However, class II beta-tubulin staining did not identify intestinal M cells in the intestines. Immunoblot analysis revealed that class II beta-tubulin expression was upregulated in HK cells, a normal FDC cell line. Class II beta-tubulin immunostaining highlighted hyperplastic FDC meshworks in all AILTs (14/14, 100%). Conclusion: Class II beta-tubulin is a specific histochemical marker for human tonsillar M cells and FDCs. Thus, class II beta-tubulin immunostaining may be useful to identify tonsillar M cells and to diagnose FDC proliferative lesions such as AILT.
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Collections - College of Medicine > Department of Medical Science > 1. Journal Articles
- Graduate School > Department of Biomedical Sciences > 1. Journal Articles
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