Cell-bound exopolysaccharide from probiotic bacteria induces autophagic cell death of tumour cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Y. | - |
dc.contributor.author | Oh, S. | - |
dc.contributor.author | Yun, H. S. | - |
dc.contributor.author | Oh, S. | - |
dc.contributor.author | Kim, S. H. | - |
dc.date.accessioned | 2021-09-08T01:25:06Z | - |
dc.date.available | 2021-09-08T01:25:06Z | - |
dc.date.created | 2021-06-14 | - |
dc.date.issued | 2010-08 | - |
dc.identifier.issn | 0266-8254 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/116021 | - |
dc.description.abstract | Aim: Lactic acid bacteria (LAB) are beneficial micro-organisms that have been associated with several probiotic effects in both humans and animals. Here, using proteome analysis, we investigate the antitumour effects of cell-bound exopolysaccharides (cb-EPS) isolated from Lactobacillus acidophilus 606 on colon cancer cells and explore the proteins critical for their antitumour activity. Methods and Results: cb-EPS inhibited the proliferation of HT-29 colon cancer cells by directly affecting cell morphology and not the cell cycle. Using two-dimensional polyacrylamide gel electrophoresis coupled with matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF/MS) and immunoblot analysis, we found that cb-EPS dramatically induced Beclin-1 and GRP78, and affected Bcl-2 and Bak regulation. Conclusions: The results of this study indicate that cb-EPS are antitumourigenic against HT-29 colon cancer cells and that this activity is because of the activation of autophagic cell death promoted directly by the induction of Beclin-1 and GRP78, as well as indirectly through the induction of Bcl-2 and Bak. Significance and Impact of the Study: These results may contribute to understanding the novel mechanisms by which probiotic bacteria induce tumour cell death via autophagy. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | WILEY | - |
dc.subject | ESCHERICHIA-COLI O157-H7 | - |
dc.subject | STRESS-INDUCED AUTOPHAGY | - |
dc.subject | EXPRESSION | - |
dc.subject | INHIBITION | - |
dc.subject | TUMORIGENESIS | - |
dc.subject | PROTEINS | - |
dc.subject | STRAINS | - |
dc.subject | ACID | - |
dc.title | Cell-bound exopolysaccharide from probiotic bacteria induces autophagic cell death of tumour cells | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, S. H. | - |
dc.identifier.doi | 10.1111/j.1472-765X.2010.02859.x | - |
dc.identifier.scopusid | 2-s2.0-77954474565 | - |
dc.identifier.wosid | 000279750600001 | - |
dc.identifier.bibliographicCitation | LETTERS IN APPLIED MICROBIOLOGY, v.51, no.2, pp.123 - 130 | - |
dc.relation.isPartOf | LETTERS IN APPLIED MICROBIOLOGY | - |
dc.citation.title | LETTERS IN APPLIED MICROBIOLOGY | - |
dc.citation.volume | 51 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 123 | - |
dc.citation.endPage | 130 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI O157-H7 | - |
dc.subject.keywordPlus | STRESS-INDUCED AUTOPHAGY | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | INHIBITION | - |
dc.subject.keywordPlus | TUMORIGENESIS | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.subject.keywordPlus | STRAINS | - |
dc.subject.keywordPlus | ACID | - |
dc.subject.keywordAuthor | antitumour activity | - |
dc.subject.keywordAuthor | autophagy | - |
dc.subject.keywordAuthor | cb-EPS | - |
dc.subject.keywordAuthor | two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.