Pegylated poly-L-arginine derivatives of chitosan for effective delivery of siRNA
- Authors
- Noh, Sang Myoung; Park, Myung Ok; Shim, Gayong; Han, Su Eun; Lee, Han Young; Huh, Jun Hyuk; Kim, Myoung Suk; Choi, Jin Joo; Kim, Kwangmeyung; Kwon, Ick Chan; Kim, Jin-Seok; Baek, Kwang-Hyun; Oh, Yu-Kyoung
- Issue Date
- 14-7월-2010
- Publisher
- ELSEVIER SCIENCE BV
- Keywords
- Chitosan; Polyarginine; Polyethylene glycol; siRNA; Serum stability
- Citation
- JOURNAL OF CONTROLLED RELEASE, v.145, no.2, pp.159 - 164
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF CONTROLLED RELEASE
- Volume
- 145
- Number
- 2
- Start Page
- 159
- End Page
- 164
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/116060
- DOI
- 10.1016/j.jconrel.2010.04.005
- ISSN
- 0168-3659
- Abstract
- For delivery of siRNA, chitosan (CS) was derivatized with poly-L-arginine (PLR) and polyethylene glycol (PEG). The formation of polyplexes with siRNA was confirmed by gel retardation. The PLR-grafted CS formed nanosized particles with siRNA. PLR-grafted CS showed higher cellular delivery efficiency of siRNA than did CS. pegylated CS, PLR, or pegylated PLR. The extent of reduction in the expression of fluorescent proteins was highest following treatment of the cells using PLR derivatives of CS in complexes with specific siRNAs. Cell viability was greater in populations treated with pegylated CS-PLR than in those treated with PLR. Hemolysis of erythrocytes was reduced upon conjugation of PLR with CS. The delivery of siRNAs via pegylated CS-PLR revealed little dependence on serum. Molecular imaging techniques revealed that the intratumoral administration of red fluorescent protein-specific siRNA in complexes with pegylated CS-PLR significantly silenced the expression of red fluorescent proteins in tumor tissues in vivo. These results indicate that pegylated CS-PLR might be useful for in vivo delivery of therapeutic siRNAs. (C) 2010 Elsevier B.V. All rights reserved.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - Graduate School > KU-KIST Graduate School of Converging Science and Technology > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.