Tumor-homing glycol chitosan/polyethylenimine nanoparticles for the systemic delivery of siRNA in tumor-bearing mice
- Authors
- Huh, Myung Sook; Lee, Seung-Young; Park, Sangjin; Lee, Seulki; Chung, Hyunjin; Lee, Sojin; Choi, Yongseok; Oh, Yu-Kyoung; Park, Jae Hyung; Jeong, Seo Young; Choi, Kuiwon; Kim, Kwangmeyung; Kwon, Ick Chan
- Issue Date
- 1-6월-2010
- Publisher
- ELSEVIER SCIENCE BV
- Keywords
- Glycol chitosan; Polyethylenimine; siRNA; Nanoparticle delivery system; Tumor-targeting delivery; Cancer treatment
- Citation
- JOURNAL OF CONTROLLED RELEASE, v.144, no.2, pp.134 - 143
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF CONTROLLED RELEASE
- Volume
- 144
- Number
- 2
- Start Page
- 134
- End Page
- 143
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/116267
- DOI
- 10.1016/j.jconrel.2010.02.023
- ISSN
- 0168-3659
- Abstract
- Here, we designed a new nano-sized siRNA carrier system composed of biocompatible/biodegradable glycol chitosan polymer (GC) and strongly positively charged polyethylenimine (PEI) polymers. In order to make a stable and tumor-homing nano-sized carrier, each polymer was modified with hydrophobic 5 beta-cholanic acid, and they were simply mixed to form self-assembled GC-PEI nanoparticles (GC-PEI NPs), due to the strong hydrophobic interactions of 5 beta-cholanic acids in the polymers. The freshly prepared GC-PEI NPs showed a stable nanoparticle structure (350 nm) and they presented a strongly positive-charged surface (zeta potential = 23.8) that is enough to complex tightly with negatively charged RFP-siRNAs, designed for inhibiting red fluorescent protein (RFP) expression. The siRNA encapsulated nanoparticles (siRNA-GC-PEI NPs) formed more compact and stable nanoparticle structures (250 nm) at 1: 5 weight ratio of siRNA to GC-PEI nanoparticles. In vitro RFP expressing B16F10 tumor cell (RFP/B16F10) culture system, the siRNA-GC-PEI NPs presented a rapid time-dependent cellular uptake profile within 1 h. Moreover, the internalized siRNA-GC-PEI NPs lead to specific mRNA breaks down. Furthermore, our new formulation of siRNA-GC-PEI NPs presented a significant inhibition of RFP gene expression of RFP/B16F10-bearing mice, due to their higher tumor-targeting ability. These results revealed the promising potential of GC-PEI NPs as a stable and effective nano-sized siRNA delivery system for cancer treatment. (C) 2010 Elsevier B.V. All rights reserved.
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Collections - Graduate School > Department of Biotechnology > 1. Journal Articles
- Graduate School > KU-KIST Graduate School of Converging Science and Technology > 1. Journal Articles
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