Expression of Membrane-Bound Mucins in Human Nasal Mucosa Different Patterns for MUC4 and MUC16

Abstract

Objective: To acquire basic information concerning the function of the membrane-bound mucin MUC16 in nasal mucosa compared with the best-characterized membrane-bound mucin, MUC4. Design: In vitro study using semiquantatitive reverse transcription polymerase chain reaction analysis and immunoassay. Setting: Yeungnam University College of Medicine. Subjects: We examined the nasal polyps obtained during endoscopic sinus surgery in 10 patients, the normal ethmoid sinus mucosa obtained from 10 patients, and human nasal polyp epithelial (HNPE) cells. Main Outcome Measures: Gene expression of MUC4 and MUC16 in nasal polyps and normal nasal mucosa. In addition, we evaluated the effect of 4 physiologically relevant agents, including retinoic acid, interleukin 113, phorbol 12-myristate 13-acetate (PMA), and dexamethasone, on the expression of MUC4 and MUC16 in HNPE cells at the gene and protein levels. Results: In nasal polyps, MUC4 was upregulated compared with normal nasal mucosa (P=.009), whereas MUC16 expression did not differ between nasal polyps and normal nasal mucosa. Retinoic acid and interleukin 113 increased MUC4 expression at the gene and protein level in HNPE cells, whereas MUC16 expression was not affected. Unlike retinoic acid and interleukin 113, PMA and dexamethasone increased MUC16 expression, whereas they had no significant effect on MUC4 expression. Conclusions: Expression of MUC4 and MUC16 are regulated differently in nasal mucosa. Dexamethasone and PMA are potent mediators for the expression of MUC16 in nasal polyps.