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Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe

Authors
Kim, Dong-UkHayles, JacquelineKim, DongsupWood, ValeriePark, Han-OhWon, MisunYoo, Hyang-SookDuhig, TrevorNam, MiyoungPalmer, GeorgiaHan, SangjoJeffery, LindaBaek, Seung-TaeLee, HyemiShim, Young SamLee, MinhoKim, LilaHeo, Kyung-SunNoh, Eun JooLee, Ah-ReumJang, Young-JooChung, Kyung-SookChoi, Shin-JungPark, Jo-YoungPark, YoungwooKim, Hwan MookPark, Song-KyuPark, Hae-JoonKang, Eun-JungKim, Hyong BaiKang, Hyun-SamPark, Hee-MoonKim, KyunghoonSong, KiwonBin Song, KyungNurse, PaulHoe, Kwang-Lae
Issue Date
6월-2010
Publisher
NATURE PUBLISHING GROUP
Keywords
schizosaccharomyces pombe
Citation
NATURE BIOTECHNOLOGY, v.28, no.6, pp.617 - U111
Indexed
SCIE
SCOPUS
Journal Title
NATURE BIOTECHNOLOGY
Volume
28
Number
6
Start Page
617
End Page
U111
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/116410
DOI
10.1038/nbt.1628
ISSN
1087-0156
Abstract
We report the construction and analysis of 4,836 heterozygous diploid deletion mutants covering 98.4% of the fission yeast genome providing a tool for studying eukaryotic biology. Comprehensive gene dispensability comparisons with budding yeast-the only other eukaryote for which a comprehensive knockout library exists-revealed that 83% of single-copy orthologs in the two yeasts had conserved dispensability. Gene dispensability differed for certain pathways between the two yeasts, including mitochondrial translation and cell cycle checkpoint control. We show that fission yeast has more essential genes than budding yeast and that essential genes are more likely than nonessential genes to be present in a single copy, to be broadly conserved and to contain introns. Growth fitness analyses determined sets of haploinsufficient and haploproficient genes for fission yeast, and comparisons with budding yeast identified specific ribosomal proteins and RNA polymerase subunits, which may act more generally to regulate eukaryotic cell growth.
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College of Pharmacy > Department of Pharmaceutical Science > 1. Journal Articles
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