Effects of the isoflavone puerarin and its glycosides on melanogenesis in B16 melanocytes
- Authors
- Choi, Young-Mi; Jun, Hee-jin; Dawson, Kevin; Rodriguez, Raymond L.; Roh, Mi Ran; Jun, Jungae; Choi, Chung-Hyo; Shim, Jae-Hoon; Lee, ChoongHwan; Lee, Sang Jun; Park, Kwan-Hwa; Lee, Sung-Joon
- Issue Date
- 5월-2010
- Publisher
- SPRINGER
- Keywords
- Puerarin; Melanogenesis; Hypopigmentation; Tyrosinase; Melanin; B16 murine melanocytes; Microarray analysis
- Citation
- EUROPEAN FOOD RESEARCH AND TECHNOLOGY, v.231, no.1, pp.75 - 83
- Indexed
- SCIE
SCOPUS
- Journal Title
- EUROPEAN FOOD RESEARCH AND TECHNOLOGY
- Volume
- 231
- Number
- 1
- Start Page
- 75
- End Page
- 83
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/116560
- DOI
- 10.1007/s00217-010-1251-5
- ISSN
- 1438-2377
- Abstract
- We investigated the effects of puerarin (8-C-glucosyl-7,4'-dihydroxy isoflavone), an isoflavone found in Kudzu roots (Pueraria lobata), and its glycosides (enzymatically synthesised, water-soluble derivatives of puerarin) on melanogenesis in vitro. Puerarin and its glycosides reduced mushroom tyrosinase activity by 88 and 67% at 4.8 mM, respectively, in a concentration-dependent manner. The puerarin glycosides were less effective than puerarin at the same concentration but showed a comparable inhibitory effect at a concentration at which puerarin is insoluble in water. In cultured B16 melanocytes, the melanin content was reduced significantly; moreover, tyrosinase activity was inhibited significantly by both puerarin and its glycosides when added at a concentration of 480 mu M. DNA microarray and RT-PCR analyses showed significant downregulation of the expression of microphthalmia-associated transcription factor (MITF) and its target genes. The protein expression of MITF and tyrosinase was also downregulated significantly by 40 and 50%, respectively. Our findings suggest that puerarin and its glycosides cause hypopigmentation via dual mechanisms: by inhibiting tyrosinase activity directly and by altering the expression of melanogenesis-related genes, such as MITF and tyrosinase. Therefore, puerarin and its glycosides may have potential for the development of functional cosmetics causing hypopigmentation.
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Collections - Graduate School > Department of Biotechnology > 1. Journal Articles
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