Improvement of Coenzyme Q(10) Production by Increasing the NADH/NAD(+) Ratio in Agrobacterium tumefaciens
DC Field | Value | Language |
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dc.contributor.author | Koo, Bong-Seong | - |
dc.contributor.author | Gong, Yun-Jung | - |
dc.contributor.author | Kim, Sang-Yong | - |
dc.contributor.author | Kim, Chan-Wha | - |
dc.contributor.author | Lee, Hyeon-Cheol | - |
dc.date.accessioned | 2021-09-08T04:07:25Z | - |
dc.date.available | 2021-09-08T04:07:25Z | - |
dc.date.created | 2021-06-11 | - |
dc.date.issued | 2010-04 | - |
dc.identifier.issn | 0916-8451 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/116696 | - |
dc.description.abstract | Previously screened CoQ(10)-overproducing Agrobacterium tumefaciens A603-35 showed a relatively high NADH/NAD(+) ratio (1.1), as compared to parental strain C58 (0.2) when we increased the expression levels of NADH-generating enzymes. Also, the intracellular NADH/NAD(+) ratio showed a positive correlation with the CoQ(10) content in A603-35. Overexpression of glyceraldehyde 3-phosphate dehydrogenase in A603-35 shifted the NADH/NAD(+) ratio at 48 h from 0.8 to 1.2, and thus the CoQ(10) content in flask culture increased from 2.16 to 3.63 mg/g DCW. Due to the addition of hydroxybutyrate to the culture media, the intracellular NADH/NAD(+) ratio in A603-35-gapA shifted from 1.2 to 1.4, which led to an increase CoQ(10) content (5.27 mg/g DCW). | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | TAYLOR & FRANCIS LTD | - |
dc.subject | DECAPRENYL DIPHOSPHATE SYNTHASE | - |
dc.subject | RECOMBINANT ESCHERICHIA-COLI | - |
dc.subject | GLUCONOBACTER-SUBOXYDANS | - |
dc.subject | MOLECULAR-CLONING | - |
dc.subject | DPS GENE | - |
dc.subject | EXPRESSION | - |
dc.subject | OVEREXPRESSION | - |
dc.subject | PATHWAY | - |
dc.title | Improvement of Coenzyme Q(10) Production by Increasing the NADH/NAD(+) Ratio in Agrobacterium tumefaciens | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Chan-Wha | - |
dc.identifier.doi | 10.1271/bbb.100034 | - |
dc.identifier.scopusid | 2-s2.0-77951523549 | - |
dc.identifier.wosid | 000277900200039 | - |
dc.identifier.bibliographicCitation | BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, v.74, no.4, pp.895 - 898 | - |
dc.relation.isPartOf | BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | - |
dc.citation.title | BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | - |
dc.citation.volume | 74 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 895 | - |
dc.citation.endPage | 898 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Food Science & Technology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Applied | - |
dc.relation.journalWebOfScienceCategory | Food Science & Technology | - |
dc.subject.keywordPlus | DECAPRENYL DIPHOSPHATE SYNTHASE | - |
dc.subject.keywordPlus | RECOMBINANT ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | GLUCONOBACTER-SUBOXYDANS | - |
dc.subject.keywordPlus | MOLECULAR-CLONING | - |
dc.subject.keywordPlus | DPS GENE | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | OVEREXPRESSION | - |
dc.subject.keywordPlus | PATHWAY | - |
dc.subject.keywordAuthor | coenzyme Q(10) | - |
dc.subject.keywordAuthor | glyceraldehyde 3-phosphate dehydrogenase | - |
dc.subject.keywordAuthor | NADH/NAD(+) ratio | - |
dc.subject.keywordAuthor | fermentation | - |
dc.subject.keywordAuthor | Agrobacterium tumefaciens | - |
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