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Improvement of Coenzyme Q(10) Production by Increasing the NADH/NAD(+) Ratio in Agrobacterium tumefaciens

Authors
Koo, Bong-SeongGong, Yun-JungKim, Sang-YongKim, Chan-WhaLee, Hyeon-Cheol
Issue Date
4월-2010
Publisher
TAYLOR & FRANCIS LTD
Keywords
coenzyme Q(10); glyceraldehyde 3-phosphate dehydrogenase; NADH/NAD(+) ratio; fermentation; Agrobacterium tumefaciens
Citation
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, v.74, no.4, pp.895 - 898
Indexed
SCIE
SCOPUS
Journal Title
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
Volume
74
Number
4
Start Page
895
End Page
898
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/116696
DOI
10.1271/bbb.100034
ISSN
0916-8451
Abstract
Previously screened CoQ(10)-overproducing Agrobacterium tumefaciens A603-35 showed a relatively high NADH/NAD(+) ratio (1.1), as compared to parental strain C58 (0.2) when we increased the expression levels of NADH-generating enzymes. Also, the intracellular NADH/NAD(+) ratio showed a positive correlation with the CoQ(10) content in A603-35. Overexpression of glyceraldehyde 3-phosphate dehydrogenase in A603-35 shifted the NADH/NAD(+) ratio at 48 h from 0.8 to 1.2, and thus the CoQ(10) content in flask culture increased from 2.16 to 3.63 mg/g DCW. Due to the addition of hydroxybutyrate to the culture media, the intracellular NADH/NAD(+) ratio in A603-35-gapA shifted from 1.2 to 1.4, which led to an increase CoQ(10) content (5.27 mg/g DCW).
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