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Enhanced humoral and cellular immune responses after sublingual immunization against human papillomavirus 16 L1 protein with adjuvants

Authors
Cho, Hee-JeongKim, Ji-YeonLee, YoungKim, Jung MoggKim, Young BongChun, TaehoonOh, Yu-Kyoung
Issue Date
19-3월-2010
Publisher
ELSEVIER SCI LTD
Keywords
Sublingual route; Mucosal vaccination; Human papillomavirus; Sublingual adjuvant
Citation
VACCINE, v.28, no.14, pp.2598 - 2606
Indexed
SCIE
SCOPUS
Journal Title
VACCINE
Volume
28
Number
14
Start Page
2598
End Page
2606
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/116790
DOI
10.1016/j.vaccine.2010.01.013
ISSN
0264-410X
Abstract
Needle-free nonparenteral vaccines offer a number of practical advantages, especially in developing countries. To address the effects of vaccine administration route, we tested mucosal and systemic immune responses against human papillomavirus 16 L1(HPV16L1) protein using intranasal, intravaginal, transdermal, sublingual (SL) and intramuscular routes. The SL route provided the most effective mucosal secretory IgA (sIgA) and serum IgG responses. After a 15014 antigen dose via the SL route, saliva sIgA levels were 7.2- and 5.8-fold higher than those achieved via intravaginal and transdermal routes, respectively. Notably, SL administration even produced 4.6-fold higher levels of vaginal sIgA levels than did intravaginal delivery of 150 mu g antigen. To enhance the immunogenicity of SL vaccines, we tested the adjuvanticity of nine molecules: three toll-like receptor agonists, three nucleotide-binding oligomerization-domain agonists, vitamin D3, poly-gamma-glutamic acid and cholera toxin subunit B (CTB). Among the molecules tested, CTB provided the most enhanced mucosal sIgA and systemic IgG induction. SL-applied CTB enhanced the production of interleukin-4 and interferon-gamma from stimulated CD4+ T cells. Moreover, interferon-gamma-producing CD8+ T cell responses were increased 1.7-fold after co-treatment with SL CTB and HPV16L1. These results suggest the potential of the SL route for delivery of HPV16L1 vaccines using CTB as an adjuvant. (C) 2010 Elsevier Ltd. All rights reserved.
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