Differential effects of 5-fluorouracil on glucose transport and expressions of glucose transporter proteins in gastric cancer cells
DC Field | Value | Language |
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dc.contributor.author | Won, Hye-Jin | - |
dc.contributor.author | Ha, Tae Kyung | - |
dc.contributor.author | Kwon, Sung Joon | - |
dc.contributor.author | Cho, Hong Yon | - |
dc.contributor.author | Hur, Sook-Jin | - |
dc.contributor.author | Baik, Hyung-Hwan | - |
dc.contributor.author | Suh, Seong-Il | - |
dc.contributor.author | Ha, Eunyoung | - |
dc.contributor.author | Kim, Yong Ho | - |
dc.date.accessioned | 2021-09-08T04:49:30Z | - |
dc.date.available | 2021-09-08T04:49:30Z | - |
dc.date.created | 2021-06-11 | - |
dc.date.issued | 2010-03 | - |
dc.identifier.issn | 0959-4973 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/116895 | - |
dc.description.abstract | Although 5-fluorouracil (5-FU) is a widely used chemotherapeutic agent in the treatment of gastric cancer, the underlying mechanism for 5-FU resistant phenotype, has yet to be elucidated. We hypothesized that the sensitivity of gastric cancer to 5-FU treatment might be related to the rate of glucose transport ( GLUT), and investigated the expressions of GLUT1, 2, 3, and 4 in two different gastric cancer cells (SNU-216, moderately differentiated gastric adenocarcinoma; and SNU-668, signet ring cell gastric carcinoma). Immunohistochemistry of GLUT1 and GLUT4 and immunoblot analysis of glycogen synthase kinase 3 were also performed. Hexokinase activity was measured. We found that 5-FU suppressed glucose uptake in SNU-216, while it stimulated GLUT in SNU-668. Further analysis revealed that 5-FU decreased the expression levels of GLUT1, 2, and 4 in SNU-216 cells and increased the expression levels of GLUT1, 2, and 4 in SNU-668 cells. Consistent with GLUT expression levels, immunohistochemistry analysis showed that 5-FU increased GLUT1 and GLUT4 levels in SNU-216 and decreased GLUT1 and GLUT4 levels in SNU-668. We also observed that glycogen synthase kinase 3 activity was decreased in SNU-216 and increased in SNU-668 with 5-FU treatment. No significant difference in hexokinase activities was observed with 5-FU treatment. Taken together, these results suggest that 5-FU exerts differential effects on GLUT depending on gastric cancer cell types, which may indicate a possible explanation, at least in part, for the differing responses to 5-FU chemotherapy in gastric cancer. Anti-Cancer Drugs 21: 270-276 (C) 2010 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | LIPPINCOTT WILLIAMS & WILKINS | - |
dc.subject | POSITRON-EMISSION-TOMOGRAPHY | - |
dc.subject | GLYCOGEN-SYNTHASE KINASE-3 | - |
dc.subject | THYMIDYLATE SYNTHASE | - |
dc.subject | SKELETAL-MUSCLE | - |
dc.subject | MESSENGER-RNA | - |
dc.subject | IN-VITRO | - |
dc.subject | CARCINOMA | - |
dc.subject | GLUT1 | - |
dc.subject | GLUCOSE-TRANSPORTER-1 | - |
dc.subject | ASSOCIATION | - |
dc.title | Differential effects of 5-fluorouracil on glucose transport and expressions of glucose transporter proteins in gastric cancer cells | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Cho, Hong Yon | - |
dc.identifier.doi | 10.1097/CAD.0b013e328334562c | - |
dc.identifier.scopusid | 2-s2.0-76649096671 | - |
dc.identifier.wosid | 000274862800006 | - |
dc.identifier.bibliographicCitation | ANTI-CANCER DRUGS, v.21, no.3, pp.270 - 276 | - |
dc.relation.isPartOf | ANTI-CANCER DRUGS | - |
dc.citation.title | ANTI-CANCER DRUGS | - |
dc.citation.volume | 21 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 270 | - |
dc.citation.endPage | 276 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Oncology | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Oncology | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.subject.keywordPlus | POSITRON-EMISSION-TOMOGRAPHY | - |
dc.subject.keywordPlus | GLYCOGEN-SYNTHASE KINASE-3 | - |
dc.subject.keywordPlus | THYMIDYLATE SYNTHASE | - |
dc.subject.keywordPlus | SKELETAL-MUSCLE | - |
dc.subject.keywordPlus | MESSENGER-RNA | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | CARCINOMA | - |
dc.subject.keywordPlus | GLUT1 | - |
dc.subject.keywordPlus | GLUCOSE-TRANSPORTER-1 | - |
dc.subject.keywordPlus | ASSOCIATION | - |
dc.subject.keywordAuthor | 5-fluorouracil | - |
dc.subject.keywordAuthor | gastric cancer | - |
dc.subject.keywordAuthor | glucose transport | - |
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