Detailed Information

Cited 0 time in webofscience Cited 0 time in scopus
Metadata Downloads

In vitro myogenic and adipogenic differentiation model of genetically engineered bovine embryonic fibroblast cell lines

Authors
Yin, JinlongJin, XunBeck, SamuelKang, Dong HoHong, ZhongshanLi, ZhehuJin, YongchengZhang, QiankunChoi, Yun-JaieKim, Sung-ChanKim, Hyunggee
Issue Date
Feb-2010
Publisher
SPRINGER
Keywords
Adipogenesis; BEFS-MyoD; BEFS-PPAR gamma 2; Bovine embryonic fibroblast cell line; Myogenesis
Citation
BIOTECHNOLOGY LETTERS, v.32, no.2, pp.195 - 202
Indexed
SCIE
SCOPUS
Journal Title
BIOTECHNOLOGY LETTERS
Volume
32
Number
2
Start Page
195
End Page
202
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/117048
DOI
10.1007/s10529-009-0142-y
ISSN
0141-5492
Abstract
Our current understanding of muscle and adipose tissue development has been largely restricted to the study of murine myogenic and adipogenic cell lines, since attempts to establish these cell lines from other species have met with only limited success. Here we report that a spontaneously immortalized bovine embryonic fibroblast cell line (BEFS) undergoes differentiation into adipogenic or myogenic lineages when ectopically transduced with PPAR gamma 2 (an adipogenic lineage determinant) or MyoD (a myogenic lineage determinant) and grown in adipogenic and myogenic differentiation culture media (ADCM and MDCM, respectively). We also found that PPAR gamma 2-overexpressing BEFS cells (BEFS-PPAR gamma 2) grown in ADCM with or without the PPAR gamma 2 ligand, troglitazone, preferentially differentiate into adipogenic cells in the presence of ectopic MyoD expression. Ectopic expression of PPAR gamma 2 in the inducible MyoD-overepxressing BEFS cells (BEFS-TetOn-MyoD) completely suppresses myogenic differentiation and leads to a significant increase in adipogenic differentiation, suggesting that the adipogenic differentiation program might be dominant. Therefore, BEFS, BEFS-PPAR gamma 2, and BEFS-TetOn-MyoD would be a valuable biological model for understanding a fundamental principle underlying myogenic and adipogenic development, and for isolating various genetic and chemical factors that enable muscle and adipocyte differentiation.
Files in This Item
There are no files associated with this item.
Appears in
Collections
College of Life Sciences and Biotechnology > Division of Biotechnology > 1. Journal Articles
Graduate School > Department of Biotechnology > 1. Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher Kim, Hyung gee photo

Kim, Hyung gee
Department of Biotechnology
Read more

Altmetrics

Total Views & Downloads

BROWSE